Rabbit alpha 1 Catenin Polyclonal Antibody | anti-CTNNA1 antibody

alpha 1 Catenin Antibody

Cat. Num. AAA31379

• Gene Names: CTNNA1; CAP102
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: alpha 1 Catenin, Antibody; alpha 1 Catenin Antibody; Alpha E-catenin; Cadherin associated protein 102kDa; Cadherin associated protein; Cadherin-associated protein; CAP 102; CAP102; Catenin (cadherin associated protein) alpha 1 102kDa; Catenin (cadherin associated protein), alpha 1, 102kDa; Catenin alpha 1; Catenin alpha-1; CTNA1_HUMAN; CTNNA 1; Ctnna1; FLJ36832; FLJ52416; MDPT2; NY REN 13 antigen; OTTHUMP00000224141; OTTHUMP00000224147; Renal carcinoma antigen NY REN 13; Renal carcinoma antigen NY-REN-13; anti-CTNNA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: alpha 1 Catenin Antibody detects endogenous levels of total alpha 1 Catenin.; Tissue Specificity: Expressed ubiquitously in normal tissues.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CTNNA1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human alpha 1 Catenin.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Sumoylated.Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion.
• Subunit Structure: Monomer and homodimer; the monomer preferentially binds to CTNNB1 and the homodimer to actin. Binds AFDN and F-actin. Possible component of an E-cadherin/ catenin adhesion complex together with E-cadherin/CDH1 and beta-catenin/CTNNB1 or gamma-catenin/JUP; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Alternatively, the CTNNA1-containing complex may be linked to F-actin by other proteins such as LIMA1. Interacts with ARHGAP21 and with AJUBA. Interacts with LIMA1 (By similarity). Interacts with vinculin/VCL. Component of an cadherin:catenin adhesion complex composed of at least of CDH26, beta-catenin/CTNNB1, alpha-catenin/CTNNA1 and p120 catenin/CTNND1.
• Similarity: Belongs to the vinculin/alpha-catenin family.
• Subcellular Location: Cytoplasm>Cytoskeleton. Cell junction>Adherens junction. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction.; Note: Found at cell-cell boundaries and probably at cell-matrix boundaries.Cell membrane>Peripheral membrane protein>Cytoplasmic side.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry)

(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry)

(At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from various samples, using alpha 1 Catenin Antibody.Lane 1: Rat liver, blocked with antigen-specific peptides,Lane 2: Rat liver,Lane 3: Hela cells(serum starvation treatment),Lane 4: HepG2 cells(heat shock treatment).)

Related Product Information for anti-CTNNA1 antibody

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 55770844
• NCBI GeneID: 1495
• NCBI Accession #: NP_001894.2
• NCBI GenBank Nucleotide #: NM_001903.2
• UniProt Accession #: P35221; Q12795
• Molecular Weight: 100,071 Da
• NCBI Official Full Name: catenin alpha-1
• NCBI Official Synonym Full Names: catenin (cadherin-associated protein), alpha 1, 102kDa
• NCBI Official Symbol: CTNNA1
• NCBI Official Synonym Symbols: CAP102
• NCBI Protein Information: catenin alpha-1; alpha-catenin; alphaE-catenin; alpha E-catenin; alpha-E-catenin; renal carcinoma antigen NY-REN-13; cadherin-associated protein,102kDa
• UniProt Protein Name: Catenin alpha-1
• UniProt Gene Name: CTNNA1
• UniProt Entry Name: CTNA1_HUMAN