Application Data
(Type 1 alpha synuclein preformed fibrils seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers . Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 uM of Type 1 alpha synuclein preformed fibrils is combined with 100 uM of alpha synuclein monomer , as compared to when 10 uM of Type 2 alpha synuclein preformed fibrils is combined with 100 uM of alpha synuclein monomer or 100 uM of alpha Synuclein monomer . Thioflavin T ex = 450 nm, em = 485 nm.)
Alpha Synuclein Active Protein | SNCA active protein
Alpha Synuclein Protein
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Applications
SDS-Page, Western Blot
Purity
Ion-exchange Purified
Synonyms
Alpha Synuclein; N/A; Alpha Synuclein Protein; Alpha synuclein monomer, Alpha-synuclein monomer, Alpha synuclein protein monomer, Alpha synuclein monomer, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NAC; SNCA active protein
Specificity
~14.46 kDa
Purity/Purification
Ion-exchange Purified
Form/Format
PBS pH7.4
Concentration
2 mg/ml (varies by lot)
Applicable Applications for SNCA active protein
Invitro Assay, SDS-PAGE, WB (Western Blot)
Nature
Recombinant
Species
Human
Expression System
E. coli
Protein Length
Full Length
Tag
No tag
Certificate of Analysis
Certified 92% pure using SDS-PAGE analysis.
Research Area(s)
Neuroscience, Neurodegeneration, Parkinson's Disease, Alzheimer's Disease
Cellular Localization
Cellular Localization, Membrane, Nucleus
Preparation and Storage
Ships on dry ice. Store at -80ºC; 1 year+ Avoid freeze/ thaw cycle.
Application Data
(Type 1 alpha synuclein preformed fibrils seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers . Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 uM of Type 1 alpha synuclein preformed fibrils is combined with 100 uM of alpha synuclein monomer , as compared to when 10 uM of Type 2 alpha synuclein preformed fibrils is combined with 100 uM of alpha synuclein monomer or 100 uM of alpha Synuclein monomer . Thioflavin T ex = 450 nm, em = 485 nm.)
Application Data
(Type 1 alpha synuclein preformed fibrils seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers . Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 uM of Type 1 alpha synuclein preformed fibrils is combined with 100 uM of alpha synuclein monomer , as compared to when 10 uM of Type 2 alpha synuclein preformed fibrils is combined with 100 uM of alpha synuclein monomer or 100 uM of alpha Synuclein monomer . Thioflavin T ex = 450 nm, em = 485 nm.)
SDS-PAGE
(SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Monomer . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (2.5 ug). Lane 3: BSA (5 ug). Lane 4: Alpha Synuclein Protein Monomer (2.5 ug) . Lane 5: Alpha Synuclein Protein Monomer (5 ug) .)
SDS-PAGE
(SDS-PAGE of ~14 kDa Human Recombinant Alpha Synuclein Protein Monomer . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (2.5 ug). Lane 3: BSA (5 ug). Lane 4: Alpha Synuclein Protein Monomer (2.5 ug) . Lane 5: Alpha Synuclein Protein Monomer (5 ug) .)
Related Product Information for SNCA active protein
Function: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson’s disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).
Product Categories/Family for SNCA active protein
References
1. "Genetics Home Reference: SNCA". US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
alpha-synuclein isoform NACP140
NCBI Official Synonym Full Names
synuclein alpha
NCBI Official Symbol
SNCA
NCBI Official Synonym Symbols
PD1; NACP; PARK1; PARK4
NCBI Protein Information
alpha-synuclein
UniProt Protein Name
Alpha-synuclein
UniProt Gene Name
SNCA
UniProt Synonym Gene Names
NACP; PARK1; NACP
UniProt Entry Name
SYUA_HUMAN
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Product Notes
The SNCA snca (Catalog #AAA103871) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alpha Synuclein can be used in a range of immunoassay formats including, but not limited to, Invitro Assay, SDS-PAGE, WB (Western Blot). Researchers should empirically determine the suitability of the SNCA snca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha Synuclein, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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