ALK-2 / ACVR1 / ALK2 recombinant protein

Recombinant Human ALK-2 / ACVR1 / ALK2 Protein (His tag)

Cat. Num. AAA173509

• Gene Names: ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
• Reactivity: Human
• Purity: >93 % as determined by reducing SDS-PAGE.
• Synonyms: ALK-2 / ACVR1 / ALK2; N/A; Recombinant Human ALK-2 / ACVR1 / ALK2 Protein (His tag); Activin Receptor Type-1; Activin Receptor Type I; ACTR-I; Activin Receptor-Like Kinase 2; ALK-2; Serine/Threonine-Protein Kinase Receptor R1; SKR1; TGF-B Superfamily Receptor Type I; TSR-I; ACVR1; ACVRLK2;ACVR1A;ACVRLK2;ALK2;FOP;SKR1; ALK-2 / ACVR1 / ALK2 recombinant protein

• Host: Baculovirus-Insect Cells
• Reactivity: Human
• Purity/Purification: >93 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 25% glycreol, 5% Trehalose, 5% Mannitol, 0.01 % Tween-80, pH 7.5.
• Sequence: Met 1-Val 124

• Predicted N Terminal: Met 21
• Species: Human
• Tag: C-His
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.; ; Shipping:; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for ALK-2 / ACVR1 / ALK2 recombinant protein

Background: ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activinin concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presentingin the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Over expression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domainthreonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP).

NCBI and Uniprot Product Information

• NCBI GI #: 4501895
• NCBI GeneID: 90
• NCBI Accession #: NP_001096.1
• NCBI GenBank Nucleotide #: NM_001105.4
• UniProt Accession #: Q04771
• Molecular Weight: Mol_Mass: 12.8 kDa; AP_Mol_Mass: 17 kDa
• NCBI Official Full Name: activin receptor type-1
• NCBI Official Synonym Full Names: activin A receptor type 1
• NCBI Official Symbol: ACVR1
• NCBI Official Synonym Symbols: FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
• NCBI Protein Information: activin receptor type-1
• UniProt Protein Name: Activin receptor type-1
• UniProt Gene Name: ACVR1
• UniProt Synonym Gene Names: ACVRLK2; ACTR-I; ALK-2; SKR1; TSR-I