Ara h 9 recombinant protein

Recombinant allergen Ara h 9 for Arachis hypogaea (Peanut)

Cat. Num. AAA72876

• Applications: ELISA, Lateral Flow, ELISA, Dot Blot, Western Blot
• Synonyms: Ara h 9; N/A; Recombinant allergen Ara h 9 for Arachis hypogaea (Peanut); Ara h 9 recombinant protein

• Host: P. pastoris
• Form/Format: Liquid solution or dry powder (lyophilised) in vials of 0.1 mg*, 1 mg and bulk * under availability, for liquid format
• Concentration: DO₂₈₀ = 0.93; A_{0.1 %} (=1 g/l) = 0.736; CONCENTRATION*: 1.26 mg/ml (varies by lot)

• Applicable Applications for Ara h 9 recombinant protein: ELISA (Indirect ELISA), LF (Lateral Flow), CLIA (Chemiluminescence Immunoassay), Positive Control, ELISA (Indirect ELISA), DB (Dot Blot), WB (Western Blot)
• Reconstitution: With 0.733 ml of sterile double- distilled water, a final concentration of 1.26 mg/ml will be obtained. The solubilization of the cake should be developed for 15 min to allow a homogeneous protein solution, considering that part of the cake can be on the glass-walls of the container. Please keep in mind that the final volume of the reconstituted protein solution may differ from the reconstitution volume mentioned in the instructions due to the hygroscopic nature of trehalose. As the protein is reconstituted, the final volume may slightly increase to reach the specified amount mentioned in the certificate of analysis. Upon reconstitution, leave the solution at least 15 min homogenizing with a mild agitation at 4°C. Avoid vigorous shaking that can cause foaming and protein denaturation. After those minutes, centrifuge the vial to ensure that all the product remains at the base and do not lose any of it on the walls. With this reconstitution, the protein will be maintained at pH 7. It is recommended that the users carry out their absorbance determinations to avoid concentration variabilities due to the equipment used, mainly in reproducibility analysis.
• Note: Proteins should be maintained frozen at high concentrations. In order to defrost the protein, maintain the aliquot at 25°C without shaking to avoid aggregation. Prior making test dilutions and after defrosting the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface.
• Preparation and Storage: Upon arrival, it should be stored at 4° to -20°C in vertical position, avoiding all possible humidity and maintaining the vials dry. Once reconstituted, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20°C to -80°C.

SDS-PAGE

(Figure 1. SDS-PAGE analysis (17%) of 4 ?l of the recombinant allergen. Purity is >95% as determined by gel electrophoresis.)

Related Product Information for Ara h 9 recombinant protein

Ara h 9 is a lipid transfer protein (LTP) and a major allergen in peanut-allergic patients from the Mediterranean region. Nonspecific lipid transfer proteins (LTPs) represent potent pollen and food allergens. Exist two Ara h 9 isoforms: Ara h 9.01 and Ara h 9.02. The two Ara h 9 isoforms displayed similar IgE reactivity. CD spectroscopy revealed similar secondary structures between Ara h 9 and Pru p 3. The Ara h 9 isoforms showed 62–68% amino acid sequence identity with Pru p 3. In peanut-allergic patients with concomitant peach allergy, Ara h 9 showed a weaker allergenic potency than Pru p 3 in histamine release assays. Therefore, Ara h 9 can induce histamine release from basophils, but to a lesser extent than Pru p 3.