SDS-PAGE
Reticuline oxidase Recombinant Protein | BBE1 recombinant protein
Recombinant Eschscholzia californica Reticuline oxidase
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Reticuline oxidase; N/A; Recombinant Eschscholzia californica Reticuline oxidase; Berberine bridge-forming enzyme; BBE; Tetrahydroprotoberberine synthase; BBE1 recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
24-538aa; Full Length of Mature Protein
Sequence
GNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGWCPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAIRGGGGGVWGAIYAWKIKLLPVPEKVTVFRVTKNVAIDEATSLLHKWQFVAEELEEDFTLSVLGGADEKQVWLTMLGFHFGLKTVAKSTFDLLFPELGLVEEDYLEMSWGESFAYLAGLETVSQLNNRFLKFDERAFKTKVDLTKEPLPSKAFYGLLERLSKEPNGFIALNGFGGQMSKISSDFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVVNNAIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMANFDYLEKTLGSDGGEVVI
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for BBE1 recombinant protein
Essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Catalyzes the stereospecific conversion of the N-methyl moiety of (S)-reticuline into the berberine bridge carbon of (S)-scoulerine.
References
Molecular cloning, expression, and induction of berberine bridge enzyme, an enzyme essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack.Dittrich H., Kutchan T.M.Proc. Natl. Acad. Sci. U.S.A. 88:9969-9973(1991) Isolation and analysis of a gene bbe1 encoding the berberine bridge enzyme from the California poppy Eschscholzia californica.Hauschild K., Pauli H.H., Kutchan T.M.Plant Mol. Biol. 36:473-478(1998) Structural and mechanistic studies reveal the functional role of bicovalent flavinylation in berberine bridge enzyme.Winkler A., Motz K., Riedl S., Puhl M., Macheroux P., Gruber K.J. Biol. Chem. 284:19993-20001(2009)
NCBI and Uniprot Product Information
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Product Notes
The BBE1 bbe1 (Catalog #AAA114386) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 24-538aa; Full Length of Mature Protein. The amino acid sequence is listed below: GNDLLSCLTF NGVRNHTVFS ADSDSDFNRF LHLSIQNPLF QNSLISKPSA IILPGSKEEL SNTIRCIRKG SWTIRLRSGG HSYEGLSYTS DTPFILIDLM NLNRVSIDLE SETAWVESGS TLGELYYAIT ESSSKLGFTA GWCPTVGTGG HISGGGFGMM SRKYGLAADN VVDAILIDAN GAILDRQAMG EDVFWAIRGG GGGVWGAIYA WKIKLLPVPE KVTVFRVTKN VAIDEATSLL HKWQFVAEEL EEDFTLSVLG GADEKQVWLT MLGFHFGLKT VAKSTFDLLF PELGLVEEDY LEMSWGESFA YLAGLETVSQ LNNRFLKFDE RAFKTKVDLT KEPLPSKAFY GLLERLSKEP NGFIALNGFG GQMSKISSDF TPFPHRSGTR LMVEYIVAWN QSEQKKKTEF LDWLEKVYEF MKPFVSKNPR LGYVNHIDLD LGGIDWGNKT VVNNAIEISR SWGESYFLSN YERLIRAKTL IDPNNVFNHP QSIPPMANFD YLEKTLGSDG GEVVI. It is sometimes possible for the material contained within the vial of "Reticuline oxidase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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