SDS-PAGE
BCKDK recombinant protein
BCKDK recombinant protein
Gene Names
BCKDK; BDK; BCKDKD
Applications
Western Blot
Purity
>95%
Synonyms
BCKDK; N/A; BCKDK recombinant protein; BCKDKD; BDK
Host
Sf9 insect cells
Purity/Purification
>95%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1ug/ul (varies by lot)
Sequence Length
2144
Applicable Applications for BCKDK recombinant protein
WB (Western Blot)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant human BCKDK (30-end) was expressed by baculovirus in Sf9 insect cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Stability: 1 yr at -70 degree C from date shipment
Storage & Shipping: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Storage & Shipping: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for BCKDK recombinant protein
Recombinant human BCKDK (30-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: BCKDK or branched chain alpha-keto acid dehydrogenase kinase is an important regulator of the valine, leucine, and isoleucine catabolic pathways. BCKDK is found in the mitochondrion where it phosphorylates and inactivates BCKD. The branched-chain alpha-keto dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the oxidation of branched-chain amino acids and BCKDK inactivates the BCKDH complex by catalyzing serine phosphorylation of the E1-alpha subunit (1). BCKDH kinase catalyzes the serine phosphorylation of the E1- alpha subunit of BCKDH (2).
Scientific Background: BCKDK or branched chain alpha-keto acid dehydrogenase kinase is an important regulator of the valine, leucine, and isoleucine catabolic pathways. BCKDK is found in the mitochondrion where it phosphorylates and inactivates BCKD. The branched-chain alpha-keto dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the oxidation of branched-chain amino acids and BCKDK inactivates the BCKDH complex by catalyzing serine phosphorylation of the E1-alpha subunit (1). BCKDH kinase catalyzes the serine phosphorylation of the E1- alpha subunit of BCKDH (2).
Product Categories/Family for BCKDK recombinant protein
References
1. Joshi, M. A. et.al: growth and neurological abnormalities in branched-chain alpha-keto acid dehydrogenase kinase-deficient mice. Biochem. J. 400: 153-162, 2006.
2. Popov, K. M. et.al: A. Branched-chain alpha-ketoacid dehydrogenase kinase: molecular cloning, expression, and sequence similarity with histidine protein kinases. J. Biol. Chem. 267: 13127-13130, 1992.
2. Popov, K. M. et.al: A. Branched-chain alpha-ketoacid dehydrogenase kinase: molecular cloning, expression, and sequence similarity with histidine protein kinases. J. Biol. Chem. 267: 13127-13130, 1992.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69 kDa
NCBI Official Full Name
Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), transcript variant 1, mRNA
NCBI Official Synonym Full Names
branched chain ketoacid dehydrogenase kinase
NCBI Official Symbol
BCKDK
NCBI Official Synonym Symbols
BDK; BCKDKD
NCBI Protein Information
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; BCKDHKIN; BCKD-kinase; branched chain alpha-ketoacid dehydrogenase kinase
UniProt Protein Name
[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial
UniProt Gene Name
BCKDK
UniProt Synonym Gene Names
BCKD-kinase; BCKDHKIN
UniProt Entry Name
BCKD_HUMAN
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Product Notes
The BCKDK bckdk (Catalog #AAA72935) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant human BCKDK (30-end) was expressed by baculovirus in Sf9 insect cells. AAA Biotech's BCKDK can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the BCKDK bckdk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCKDK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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