B-Cell Leukemia/Lymphoma 2 -BH3 Recombinant Protein | Bcl-2 -BH3 recombinant protein
Recombinant Human B-Cell Leukemia/Lymphoma 2 -BH3
Gene Names
BCL2; Bcl-2; PPP1R50
Applications
Western Blot
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
B-Cell Leukemia/Lymphoma 2 -BH3; N/A; Recombinant Human B-Cell Leukemia/Lymphoma 2 -BH3; Bcl 2 Human (minus BH3 domain); B-Cell Leukemia/Lymphoma 2 Human Recombinant (BH3); Apoptosis regulator Bcl-2; BCL2; B-cell CLL/lymphoma 2; Bcl-2; Bcl-2 -BH3 recombinant protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence Length
239
Applicable Applications for Bcl-2 -BH3 recombinant protein
WB (Western Blot)
Solubility
Suspend Bcl-2 in 100ul of 0.5M Acetic acid, over night at 4 degree C. Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Preparation and Storage
Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Bcl-2 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for Bcl-2 -BH3 recombinant protein
Description: Bcl-2 Des BH3 domain (93-107 residues) Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 203 amino acids 1-92 and 108-218.The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.
Introduction: BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Introduction: BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Product Categories/Family for Bcl-2 -BH3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,337 Da
NCBI Official Full Name
apoptosis regulator Bcl-2 alpha isoform
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 2
NCBI Official Symbol
BCL2
NCBI Official Synonym Symbols
Bcl-2; PPP1R50
NCBI Protein Information
apoptosis regulator Bcl-2; protein phosphatase 1, regulatory subunit 50
UniProt Protein Name
Apoptosis regulator Bcl-2
UniProt Gene Name
BCL2
UniProt Entry Name
BCL2_HUMAN
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Product Notes
The Bcl-2 -BH3 bcl2 (Catalog #AAA38622) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's B-Cell Leukemia/Lymphoma 2 -BH3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the Bcl-2 -BH3 bcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B-Cell Leukemia/Lymphoma 2 -BH3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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