Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CCR6 at 10ug/mL can bind Anti-CCR6 recombinant antibody , the EC50 is 44.79-56.10 ng/mL.VLPs is negative control.)
C-C chemokine receptor type 6 (CCR6) Recombinant Protein | CCR6 recombinant protein
Recombinant Human C-C chemokine receptor type 6 (CCR6)-VLPs (Active)
Gene Names
CCR6; BN-1; DCR2; DRY6; CCR-6; CD196; CKRL3; GPR29; CKR-L3; CMKBR6; GPRCY4; STRL22; CC-CKR-6; C-C CKR-6
Purity
The purity information is not available for VLPs proteins.
Synonyms
C-C chemokine receptor type 6 (CCR6); N/A; Recombinant Human C-C chemokine receptor type 6 (CCR6)-VLPs (Active); CKRL3; CMKBR6; GPR29; STRL22; CCR6 recombinant protein
Host
Mammalian cell
Purity/Purification
The purity information is not available for VLPs proteins.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Sequence Positions
1-374aa; Full Length
Sequence
MSGESMNFSDVFDSSEDYFVSVNTSYYSVDSEMLLCSLQEVRQFSRLFVPIAYSLICVFGLLGNILVVITFAFYKKARSMTDVYLLNMAIADILFVLTLPFWAVSHATGAWVFSNATCKLLKGIYAINFNCGMLLLTCISMDRYIAIVQATKSFRLRSRTLPRSKIICLVVWGLSVIISSSTFVFNQKYNTQGSDVCEPKYQTVSEPIRWKLLMLGLELLFGFFIPLMFMIFCYTFIVKTLVQAQNSKRHKAIRVIIAVVLVFLACQIPHNMVLLVTAANLGKMNRSCQSEKLIGYTKTVTEVLAFLHCCLNPVLYAFIGQKFRNYFLKILKDLWCVRRKYKSSGFSCAGRYSENISRQTSETADNDNASSFTM
Species
Homo sapiens (Human)
Tag
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Research Area
CancerLess than 1.0 EU/ug as determined by LAL method.
Transmembrane Domain
7TM
Biological_Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CCR6 at 10 ug/mL can bind Anti-CCR6 recombinant antibody. The EC50 is 44.79-56.10 ng/mL.The VLPs is negative control.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CCR6 at 10ug/mL can bind Anti-CCR6 recombinant antibody , the EC50 is 44.79-56.10 ng/mL.VLPs is negative control.)
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CCR6 at 10ug/mL can bind Anti-CCR6 recombinant antibody , the EC50 is 44.79-56.10 ng/mL.VLPs is negative control.)
Application Data
(is detected by Mouse anti-6*His monoclonal antibody. The two bands respectively correspond to monomer, Homodimer.)
Application Data
(is detected by Mouse anti-6*His monoclonal antibody. The two bands respectively correspond to monomer, Homodimer.)
Related Product Information for CCR6 recombinant protein
Act as a receptor for the C-C type chemokine CCL20.Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels.Act as a receptor for non-chemokine ligands such as beta-defensins.
Product Categories/Family for CCR6 recombinant protein
References
"Identification of CCR6, the specific receptor for a novel lymphocyte- directed CC chemokine LARC."Baba M., Imai T., Nishimura M., Kakizaki M., Takagi S., Hieshima K., Nomiyama H., Yoshie O.J. Biol. Chem. 272:14893-14898 (1997)"Molecular cloning and RNA expression of two new human chemokine receptor- like genes."Zaballos A., Varona R., Gutierrez J., Lind P., Marquez G.Biochem. Biophys. Res. Commun. 227:846-853 (1996)"Cloning of STRL22, a new human gene encoding a G-protein-coupled receptor related to chemokine receptors and located on chromosome 6q27."Liao F., Lee H.-H., Farber J.M.Genomics 40:175-180 (1997)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,494 Da
NCBI Official Full Name
C-C chemokine receptor type 6
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 6
NCBI Official Symbol
CCR6
NCBI Official Synonym Symbols
BN-1; DCR2; DRY6; CCR-6; CD196; CKRL3; GPR29; CKR-L3; CMKBR6; GPRCY4; STRL22; CC-CKR-6; C-C CKR-6
NCBI Protein Information
C-C chemokine receptor type 6; LARC receptor; chemokine receptor-like 3; chemokine (C-C) receptor 6; G protein-coupled receptor 29; G-protein coupled receptor 29; seven-transmembrane receptor, lymphocyte, 22
UniProt Protein Name
C-C chemokine receptor type 6
UniProt Gene Name
CCR6
UniProt Synonym Gene Names
CKRL3; CMKBR6; GPR29; STRL22; C-C CKR-6; CC-CKR-6; CCR-6; CKR-L3; GPRCY4
UniProt Entry Name
CCR6_HUMAN
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Product Notes
The CCR6 ccr6 (Catalog #AAA279257) is a Recombinant Protein produced from Mammalian cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-374aa; Full Length. The amino acid sequence is listed below: MSGESMNFSD VFDSSEDYFV SVNTSYYSVD SEMLLCSLQE VRQFSRLFVP IAYSLICVFG LLGNILVVIT FAFYKKARSM TDVYLLNMAI ADILFVLTLP FWAVSHATGA WVFSNATCKL LKGIYAINFN CGMLLLTCIS MDRYIAIVQA TKSFRLRSRT LPRSKIICLV VWGLSVIISS STFVFNQKYN TQGSDVCEPK YQTVSEPIRW KLLMLGLELL FGFFIPLMFM IFCYTFIVKT LVQAQNSKRH KAIRVIIAVV LVFLACQIPH NMVLLVTAAN LGKMNRSCQS EKLIGYTKTV TEVLAFLHCC LNPVLYAFIG QKFRNYFLKI LKDLWCVRRK YKSSGFSCAG RYSENISRQT SETADNDNAS SFTM. It is sometimes possible for the material contained within the vial of "C-C chemokine receptor type 6 (CCR6), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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