Application Data
CES1 recombinant protein
CES1 Protein, Human, Recombinant (His Tag), HPLC-verified
Gene Names
CES1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
Purity
>= 95% as determined by SDS-PAGE.= 90% as determined by SEC-HPLC.
Synonyms
CES1; N/A; CES1 Protein, Human, Recombinant (His Tag), HPLC-verified; carboxylesterase 1; ACAT Protein, Human; CE-1 Protein, Human; CEH Protein, Human; CES2 Protein, Human; hCE-1 Protein, Human; HMSE Protein, Human; HMSE1 Protein, Human; PCE-1 Protein, Human; REH Protein, Human; SES1 Protein, Human; TGH Protein, Human; CES1 recombinant protein
Host
HEK293 Cells
Purity/Purification
>= 95% as determined by SDS-PAGE.
= 90% as determined by SEC-HPLC.
= 90% as determined by SEC-HPLC.
Form/Format
Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Species
Human
Predicted N Terminal
Gly 18
Endotoxin
<1.0 EU per ug protein as determined by the LAL method.
Activity
Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >10,000 pmol/min/ug.
Protein Construction
A DNA sequence encoding the Human CES1 (NP_001020365)(Met1-Leu567) was expressed with a polyhistidine tag at the C-terminus.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for CES1 recombinant protein
CES1 encodes carboxylesterase-1, an important drug-metabolizing enzyme with high expression in the liver. Variants generated by the exchange of segments with CES1P1 complicate the genotyping of CES1. CES1 is involved in the hydrolysis of ester group-containing xenobiotic and endobiotic compounds including several essential and commonly used drugs. The individual variation in the efficacy and tolerability of many drugs metabolized by CES1 is considerable. However, it has been problematic to express recombinant CES1 in bacterial expression systems due to low solubility, with the CES1 protein being mainly expressed in inclusion bodies, accompanied by insufficient purity issues. CES1 is capable of catalyzing the hydrolysis of a wide range of therapeutic agents, toxins, and endogenous compounds. Accumulating studies have demonstrated associations between the expression and activity of CES1 and the pharmacokinetics and/or pharmacodynamics of CES1 substrate medications.
References
Sanford JC, et al. (2016) Regulatory effects of genomic translocations at the human carboxylesterase-1 (ces1) gene locus. Pharmacogenet Genomics 26 (5): 197-207.Rasmussen HB, et al. (2015) Individualization of treatments with drugs metabolized by ces1: Combining genetics and metabolomics. Pharmacogenomics 16 (6): 649-665.Qian Y, et al. (2020) Natural products as modulators of ces1 activity. Drug Metab Dispos 48 (10): 993-1007.Ferrero-Miliani L, et al. (2017) Reappraisal of the genetic diversity and pharmacogenetic assessment of ces1. Pharmacogenomics 18 (13): 1241-1257.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61kDa
NCBI Official Full Name
liver carboxylesterase 1 isoform b
NCBI Official Synonym Full Names
carboxylesterase 1
NCBI Official Symbol
CES1
NCBI Official Synonym Symbols
CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1
NCBI Protein Information
liver carboxylesterase 1
UniProt Protein Name
Liver carboxylesterase 1
UniProt Gene Name
CES1
UniProt Synonym Gene Names
CES2; SES1; ACAT; CE-1; hCE-1; REH; TGH
UniProt Entry Name
EST1_HUMAN
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Product Notes
The CES1 ces1 (Catalog #AAA259212) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CES1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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