Activin Active Protein | INHBA active protein

Human Activin Recombinant (CHO Cell)

Cat. Num. AAA76389

• Gene Names: INHBA; EDF; FRP
• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: Activin; N/A; Human Activin Recombinant (CHO Cell); INHBA active protein

• Host: Chinese Hamster Ovary cell line (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Human Activin A was lyophilized from a 0.2 um filtered solution in 0.2M AcOH, pH 4.0.
• Sequence: MPLLWLRGFL LASCWIIVRS SPTPGSEGHS AAPDCPSCAL AALPKDVPNS 50 QPEMVEAVKK HILNMLHLKK RPDVTQPVPK AALLNAIRKL HVGKVGENGY 100 VEIEDDIGRR AEMNELMEQT SEIITFAESG TARKTLHFEI SKEGSDLSVV 150 ERAEVWLFLK VPKANRTRTK VTIRLFQQQK HPQGSLDTGE EAEEVGLKGE 200 RSELLLSEKV VDARKSTWHV FPVSSSIQRL LDQGKSSLDV RIACEQCQES 250 GASLVLLGKK KKKEEEGEGK KKGGGEGGAG ADEEKEQSHR PFLMLQARQS 300 EDHPHRRRRR GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC 350 EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS 400 MLYYDDGQNI IKKDIQNMIV EECGCS 426

• Source: Optimized DNA sequence encoding Human Inhibin Beta Alpha Chain was expressed in Chinese Hamster Ovary cell line.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Biological Activity: The ED(50) was determined by the dose-dependent proliferation ofmurine MCP11 cell line was found to be in the les than 1 ng/ml.
• Method: Isolation, culture and 48 h induction of neural stem cellsFor 48 h induction using serum-free conditions with growth factors, dissociated neurospheres were cultured with recombinant human (rh) BMP4 250 ng/mL, rh Activin A 100 ng/mL and rh bFGF 100 ng/mL individually as well as in combinations as indicated in Supporting Information Fig S1 andBlimp1-/- EpiSC Derivation and CultureEpiSCs were derived from E6.5 epiblasts by culturing on MEFs in medium'>N2B27 medium containing human activin A (20 ng/ml), bFGF (12 ng/ml), and KSR (20%) (Human ES cell culture and differentiationHuman H1, H9 and UCLA1-6 (UCLA stem cell core) [-1 bFGF.; EBs were differentiated according to previous reports with minor modification [uM Y27632 (Rho kinase inhibitor IV, EMD Chemicals), 3 ng ml-1 Activin A, 5 ng ml-1 bFGF, 10 ng ml-1 BMP4 and 5 uM IWR-1 (EMD Chemicals), for the first 4 days.; EBs were then cultured in StemPro 34 supplemented with 10 ng ml-1 bFGF, 5 ng ml-1 vascular endothelial growth factor (VEGF) and 5 uM IWR-1 for 5 additional days before plating onto elastic substrates.; Usage of all human ES cell lines is approved by the UCLA Embryonic Stem Cell Research Oversight (ESCRO) Committee and the Institutional Review Boards (IRB, approval #2009-006-04).Differentiation of human ESC and iPSC into definitive endodermDifferentiation into definitive endoderm was performed as previously described.; Briefly, pluripotent stem cells were harvested, gently triturated into single cell suspensions and seeded onto transwells (0.4um pore size, Corning) pre-coated with human placental collagen Type IV, which has previously been shown to support airway epithelial cell growth.; The cells were immediately treated with 100ng/ml Activin-A and 25ng/ml WNT3A for 4 consecutive days in Endoderm; ifferentiation Media consisting of serum-free Knockout MEM with Glutamax, penicillin/streptomycin (GIBCO), 1 mM nonessential amino acids (GIBCO) and 0.5 mM mercaptoethanol.; Subsequent differentiation steps were performed on the transwells.
• Molecular Function: Growth-factor, Hormone
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Application Data

(WNT3 Is a Functional Biomarker for Predicting the DE Differentiation Potential (A) WNT3 levels in control and various knockdown HUES8 sublines correlate with their DE differentiation efficiency.The four protocols included the following treatments in a serum-free medium: (1) A - > A (Activin A only for 4 days); (2) AW - > A (Activin A and Wnt3a for 2 days followed by Activin A only for another 2 days); (3) A - > AX (Activin A only for 2 days followed by Activin A and XAV 939 for another 2 days); and (4) AW - > AX (Activin A and Wnt3a for 2 days followed by Activin A and XAV 939 for another 2 days).)

Application Data

(Differentiation from human iPS cells toward hepatic lineage cells.Human iPS cells were sequentially stimulated with various cytokines: (1) activin A, (2) basic FGF and BMP-4, and (3) HGF.)

Application Data

(K02288 selectively inhibits BMP signaling.)

Application Data

(Loss of REX1 within the pluripotent population primes cells for differentiation.n?=?3 E) Fold enrichment of the percentage of GATA4 positive endoderm cells generated from TRA+VEN? cells relative to those from TRA+VEN+ population after 3 days of treatment with Activin A and BMP4 in low serum media, as observed by immunocytochemistry.)

Application Data

(Directed differentiation of hESC-derived cysts to RPE using transwell filters.Ac+/Ac?: with or without Activin A.)

Product Categories/Family for INHBA active protein

Cytokines; Mouse reagents; Recombinant Mouse Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4504699
• NCBI GeneID: 3624
• NCBI Accession #: NP_002183.1
• NCBI GenBank Nucleotide #: NM_002192.2
• UniProt Accession #: P08476
• Molecular Weight: 426
• NCBI Official Full Name: inhibin beta A chain
• NCBI Official Synonym Full Names: inhibin, beta A
• NCBI Official Symbol: INHBA
• NCBI Official Synonym Symbols: EDF; FRP
• NCBI Protein Information: inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypepti
• UniProt Protein Name: Inhibin beta A chain
• UniProt Gene Name: INHBA
• UniProt Synonym Gene Names: EDF
• UniProt Entry Name: INHBA_HUMAN