Angiopoietin-2 / Ang-2 Active Protein | Ang-2 active protein

Human Angiopoietin-2 / Ang-2

Cat. Num. AAA14953

• Gene Names: VCAN; WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
• Reactivity: Human
• Applications: Immunoblot, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA
• Purity: > 95%, by SDS-PAGE and HPLC analysis
• Synonyms: Angiopoietin-2 / Ang-2; N/A; Human Angiopoietin-2 / Ang-2; ANGPT2; ANG2; AGPT2; Ang-2 active protein

• Host: CHO Cells
• Reactivity: Human
• Purity/Purification: > 95%, by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized (10mM Sodium Phosphate, pH 8.0)
• Sequence: DAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQ QNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE L QLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHII QLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QH DLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTN GI YTLTFPNSTE EIKAYCDMEA GGGGWTIIQR REDGSVDFQR TWK EYKVGFG NPSGEYWLGN EFVSQLTNQQ RYVLKIHLKD WEGNEAYSL Y EHFYLSSEEL NYRIHLKGLT GTAGKISSIS QPGNDFSTKD GDND KCICKC SQMLTGGWWF DACGPSNLNG MYYPQRQNTN KFNGIKWYYW KGSGYSLKAT TMMIRPADFH HHHHH
• Sequence Length: 435

• Applicable Applications for Ang-2 active protein: Immunoblotting (IB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Sandwich ELISA (EIA)
• Application Notes: IB: 1-10ug/ml; IHC: 1-10ug/ml; IF: 1-10ug/ml; IP: 1-10ug/ml; EIA (Sandwich): Sandwich ELISA (works equally well as capture or detection antibody). ; ; These are recommended concentrations; enduser should determine optimal concentrations for their applications.
• Length (aa): 435
• Gene-ID: 285
• mRNA RefSeq: NM_001147.2
• Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug)
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Tag Information: His-Tag
• Biological Activity: Determined by its ability to stimulate tubulogenesis in HUVEC cells using concentration of 0.2ug/ml.
• Preparation and Storage: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C. Avoid repeated freeze and thaw cycles!

Related Product Information for Ang-2 active protein

Human Angiopoitin-2 (Ang-2), a 66 kDa protein consisting of 476 amino acid residues (N21-F496), is fused to a N-terminal myc-tag and produced in insect cells. The angiopoietin (Ang) family of growth factors includes four members, all of which bind to the endothelial receptor tyrosine kinase Tie2. Two of the Angs, Ang-1 and Ang-4, activate the Tie2 receptor, whereas Ang-2 and Ang-3 inhibit Ang-1-induced Tie2 phosphorylation. Angiopoietin-1 (Ang-1) is a secreted growth factor which binds to and activates the Tie-2 receptor tyrosine kinase. The factor enhances endothelial cell survival and capillary morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but fails to activate it: hence, it is a natural inhibitor of Ang-1. Ang-2 destabilises capillary integrity, facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high, but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but its ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalian metanephros, the precursor of the adult kidney, where they may play a role in endothelial precursor growth. Tie-1-expressing cells can be detected in the metanephros when it first forms and, based on transplantation experiments, these precursors contribute to the generation of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-1 and mesangial-cell-derived Ang-2 may affect growth of nascent capillaries. After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descending limbs of loops of Henle would be well placed to affect the growth of this medullary microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vessel growth in Wilms\' kidney tumours and in vascular remodelling after nephrotoxicity. Altogether, existing data suggest that VEGF-A and Angiopoietins not only have quite different roles during vascular development, but also very complementary and coordinated roles.

Product Categories/Family for Ang-2 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 187607300
• NCBI GeneID: 1462
• NCBI Accession #: NP_001138
• NCBI GenBank Nucleotide #: NM_001126336.2
• UniProt Accession #: Q15123; P20754; Q13010; Q13189; Q9UCL9; Q9UNW5; P13611
• Molecular Weight: 60-70 kDa
• NCBI Official Full Name: versican core protein isoform 2
• NCBI Official Synonym Full Names: versican
• NCBI Official Symbol: VCAN
• NCBI Official Synonym Symbols: WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
• NCBI Protein Information: versican core protein; versican proteoglycan; large fibroblast proteoglycan; glial hyaluronate-binding protein; chondroitin sulfate proteoglycan 2; chondroitin sulfate proteoglycan core protein 2
• UniProt Protein Name: Versican core protein
• UniProt Gene Name: VCAN
• UniProt Synonym Gene Names: CSPG2; Chondroitin sulfate proteoglycan 2; GHAP
• UniProt Entry Name: CSPG2_HUMAN