Application Data
(Figure 3: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the CHO-K1/ACE2 stable cell line. CHO-K1/ACE2 cells were incubated with various concentrations of biotinylated SARS-Cov-2 Spike RBD protein and analyzed through In-Cell ELISA.)
COVID 19 Spike RBD Coronavirus Recombinant Protein | COVID-19 recombinant protein
Biotinylated SARS-Cov-2 Spike RBD Protein Fc Tag (319-541aa)
Applications
Functional Assay
Purity
>=95% by SDS-PAGE
Synonyms
COVID 19 Spike RBD Coronavirus; N/A; Biotinylated SARS-Cov-2 Spike RBD Protein Fc Tag (319-541aa); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike RBD; Receptor Binding Domain; COVID-19 recombinant protein
Host
CHO cells
Purity/Purification
>=95% by SDS-PAGE
Form/Format
Recombinant protein is supplied at 0.5mg/ml in PBS, pH 7.4 with glycerol
Sequence Positions
319-541aa
Applicable Applications for COVID-19 recombinant protein
FA (Functional Assay)
Preparation and Storage
Store the recombinant protein at 4 degree C, stable for 6 months. Avoid repeated freeze and thaw cycles
Application Data
(Figure 3: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the CHO-K1/ACE2 stable cell line. CHO-K1/ACE2 cells were incubated with various concentrations of biotinylated SARS-Cov-2 Spike RBD protein and analyzed through In-Cell ELISA.)
Application Data
(Figure 3: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the CHO-K1/ACE2 stable cell line. CHO-K1/ACE2 cells were incubated with various concentrations of biotinylated SARS-Cov-2 Spike RBD protein and analyzed through In-Cell ELISA.)
Application Data
(Figure 2: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the CHO-K1/ACE2 stable cell line. CHO-K1/ACE2 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein and analyzed by flow cytometry through fluorescent-labeled Streptavidin detection.)
Application Data
(Figure 2: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the CHO-K1/ACE2 stable cell line. CHO-K1/ACE2 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein and analyzed by flow cytometry through fluorescent-labeled Streptavidin detection.)
Application Data
(Figure 1: ACE2 (human)(rec.) binds with high affinity to the biotinylated Spike (RBD) protein of the virus SARS-CoV-2.)
Application Data
(Figure 1: ACE2 (human)(rec.) binds with high affinity to the biotinylated Spike (RBD) protein of the virus SARS-CoV-2.)
Related Product Information for COVID-19 recombinant protein
Receptor-binding domain (RBD) of SARS-CoV-2 Spike protein S1 (aa 319-541) is fused with the Fc region of human IgG1 at C-terminus. Amino acid sequence was derived from Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome.
SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. The coronaviral genome encodes four major structural proteins: the Spike (S) protein, Nucleocapsid (N) protein, Membrane/Matrix (M) protein and the Envelope (E) protein. The SARS Envelope (E) protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. The SARS Membrane/Matrix (M) protein is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with SARS Spike (S) protein and the Nucleocapsid (N) protein. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms.
The CoV Spike (S) protein assembles as trimer and plays the most important role in viral attachment, fusion and entry. It is composed of a short intracellular tail, a transmembrane anchor and a large ectodomain that consists of a receptor binding S1 subunit (RBD domain) and a membrane-fusing S2 subunit. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor angiotensin-converting enzyme 2 (ACE2) present at the surface of epithelial cells.
SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. The coronaviral genome encodes four major structural proteins: the Spike (S) protein, Nucleocapsid (N) protein, Membrane/Matrix (M) protein and the Envelope (E) protein. The SARS Envelope (E) protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. The SARS Membrane/Matrix (M) protein is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with SARS Spike (S) protein and the Nucleocapsid (N) protein. The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms.
The CoV Spike (S) protein assembles as trimer and plays the most important role in viral attachment, fusion and entry. It is composed of a short intracellular tail, a transmembrane anchor and a large ectodomain that consists of a receptor binding S1 subunit (RBD domain) and a membrane-fusing S2 subunit. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor angiotensin-converting enzyme 2 (ACE2) present at the surface of epithelial cells.
Product Categories/Family for COVID-19 recombinant protein
NCBI and Uniprot Product Information
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Product Notes
The COVID-19 (Catalog #AAA78553) is a Recombinant Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 319-541aa. AAA Biotech's COVID 19 Spike RBD Coronavirus can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay). Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike RBD Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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