Cytotoxic T-lymphocyte Protein 4 Recombinant Protein | CTLA4 recombinant protein

Recombinant Human Cytotoxic T-lymphocyte Protein 4 Protein (C-DYKDDDDK)

Cat. Num. AAA174014

• Gene Names: CTLA4; CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Synonyms: Cytotoxic T-lymphocyte Protein 4; N/A; Recombinant Human Cytotoxic T-lymphocyte Protein 4 Protein (C-DYKDDDDK); Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CTLA4; CTLA4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 95 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution of 20mM PB, 50mM Nacl, 8% Trehalose, 4% Mannitol, 0.02% Tween 80, pH 6.6
• Sequence Positions: Lys36-Asp161
• Sequence Length: 174

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL method
• Tag: C-Flag
• Sequence: Lys36-Asp161
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Reconstitution: We recommend centrifuging the vial before opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C.; Reconstituted protein solution can be stored at 4-8 degree for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months

WB (Western Blot)

Related Product Information for CTLA4 recombinant protein

Cytotoxic Tlymphocyte 4(CTLA-4, CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

NCBI and Uniprot Product Information

• NCBI GI #: 83700231
• NCBI GeneID: 1493
• NCBI Accession #: NP_001032720.1
• NCBI GenBank Nucleotide #: NM_001037631.3
• UniProt Accession #: P16410; O95653; Q0PP65; Q52MC1; Q53TD5; Q5S005; Q8WXJ1; Q96P43; Q9UKN9; A0N1S0; E9PDH0
• Molecular Weight: Molecular Mass: 14.5 kDa; Actual Protein Molecular Mass: 20 kDa
• NCBI Official Full Name: cytotoxic T-lymphocyte protein 4 isoform CTLA-4delTM
• NCBI Official Synonym Full Names: cytotoxic T-lymphocyte associated protein 4
• NCBI Official Symbol: CTLA4
• NCBI Official Synonym Symbols: CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
• NCBI Protein Information: cytotoxic T-lymphocyte protein 4
• UniProt Protein Name: Cytotoxic T-lymphocyte protein 4
• UniProt Gene Name: CTLA4
• UniProt Synonym Gene Names: CD152; CTLA-4