SDS-PAGE
(Purity: Sample purity data. For lot specific data, please refer to the respective datasheet.)
DGKK recombinant protein
DGKK, Active
Synonyms
DGKK; N/A; DGKK, Active; Diacylglycerol kinase kappa; DGK-kappa; DGKK recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample purity data. For lot specific data, please refer to the respective datasheet.)
SDS-PAGE
(Purity: Sample purity data. For lot specific data, please refer to the respective datasheet.)
Activity
(Activity: Sample activity data. For lot specific data, please refer to the respective datasheet.)
Activity
(Activity: Sample activity data. For lot specific data, please refer to the respective datasheet.)
Related Product Information for DGKK recombinant protein
Recombinant full-length human DGKK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NP_001013764.1.
Diacylglycerol kinase kappa (DGKK) is a member of the type II diacylglycerol kinase subfamily. Diacylglycerol kinases modulate the concentration of intracellular diacylglycerol by phosphorylating, producing phosphatidic acid (PA)(1). PA regulates signaling proteins, such as protein kinases and phosphatases, and can induce cell mitosis through regulation of growth factors (2). Genetic variants of DGKk are associated with hypospadias in mild and moderate Han Chinese cases (3).
Diacylglycerol kinase kappa (DGKK) is a member of the type II diacylglycerol kinase subfamily. Diacylglycerol kinases modulate the concentration of intracellular diacylglycerol by phosphorylating, producing phosphatidic acid (PA)(1). PA regulates signaling proteins, such as protein kinases and phosphatases, and can induce cell mitosis through regulation of growth factors (2). Genetic variants of DGKk are associated with hypospadias in mild and moderate Han Chinese cases (3).
Product Categories/Family for DGKK recombinant protein
References
1. Imai, S. et al: Identification and characterization of a novel human type II diacylglycerol kinase, DGK kappa. J Biol Chem. 2005; 280(48):39870-81.
2. Buckland, A.G. et al: Anionic phospholipids, interfacial binding and the regulation of cell functions. Biochim Biophys Acta. 2000; 1483(2):199-216.
3. Xie, H. et al: Association between diacylglycerol kinase kappa variants and hypospadias susceptibility in a Han Chinese population. Asian journal of andrology. 2018; 20 (1): 85-89.
2. Buckland, A.G. et al: Anionic phospholipids, interfacial binding and the regulation of cell functions. Biochim Biophys Acta. 2000; 1483(2):199-216.
3. Xie, H. et al: Association between diacylglycerol kinase kappa variants and hypospadias susceptibility in a Han Chinese population. Asian journal of andrology. 2018; 20 (1): 85-89.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
141,829 Da
NCBI Official Full Name
diacylglycerol kinase kappa
NCBI Official Synonym Full Names
diacylglycerol kinase, kappa
NCBI Official Symbol
DGKK
NCBI Protein Information
diacylglycerol kinase kappa; DGK-kappa; DAG kinase kappa; diglyceride kinase kappa; 142 kDa diacylglycerol kinase
UniProt Protein Name
Diacylglycerol kinase kappa
UniProt Gene Name
DGKK
UniProt Synonym Gene Names
DAG kinase kappa; DGK-kappa
UniProt Entry Name
DGKK_HUMAN
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Product Notes
The DGKK dgkk (Catalog #AAA73421) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "DGKK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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