WB (Western Blot)
(Sample: Active recombinant AST, Human)
Aspartate Aminotransferase (AST) Active Protein | AST active protein
Active Aspartate Aminotransferase (AST)
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Sample: Active recombinant AST, Human)
WB (Western Blot)
(Sample: Active recombinant AST, Human)
SDS_PAGE
(SDS-PAGE)
SDS_PAGE
(SDS-PAGE)
Identification
(Gene Sequencing (extract))
Identification
(Gene Sequencing (extract))
Bioactivity
(Aspartate transaminase(AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or glutamic oxaloacetic transaminase, is a pyridoxal phosphate-dependent transaminase enzyme AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate and, as such, is an important enzyme in amino acid metabolism. AST is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. In this test, an amino group is transferred of from aspartate to -ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The oxaloacetic acid can be decomposed into pyruvate and carbon dioxide with the present of phenylamine citrate. The activity of aspartate transaminase can be measured by calculating the concentration of the pyruvate. The reaction was performed in adding 10ul different concentation recombinant AST(the blank tube add 10ul phosphate buffer) to 50ul mixture substrate contianing 2mM 2-Ketoglutaric acid, 0.1M L-aspartic acid, in 0.2M phosphate buffer,pH7.4, incubate at 37°C for 1h, then add 10ul phenylamine citrate and 50ul 2,4-dinitrophenylhydrazine continue incubate at 37°C for 20min, stop the action by adding 500ul 0.4M NaOH, read the OD value at 520nm. Standard curve prepare by double dilute 2uM pyruvate with phosphate bufferr then add 10ul phenylamine citrate and 50ul 2,4-dinitrophenylhydrazine, incubate at 37°C for 20min and record the OD value at 520nm. One unit of AST is the amount of enzyme that will generate 1umole of pyruvate per minute at pH7.4 at 37°C.)
Bioactivity
(Aspartate transaminase(AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or glutamic oxaloacetic transaminase, is a pyridoxal phosphate-dependent transaminase enzyme AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate and, as such, is an important enzyme in amino acid metabolism. AST is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. In this test, an amino group is transferred of from aspartate to -ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The oxaloacetic acid can be decomposed into pyruvate and carbon dioxide with the present of phenylamine citrate. The activity of aspartate transaminase can be measured by calculating the concentration of the pyruvate. The reaction was performed in adding 10ul different concentation recombinant AST(the blank tube add 10ul phosphate buffer) to 50ul mixture substrate contianing 2mM 2-Ketoglutaric acid, 0.1M L-aspartic acid, in 0.2M phosphate buffer,pH7.4, incubate at 37°C for 1h, then add 10ul phenylamine citrate and 50ul 2,4-dinitrophenylhydrazine continue incubate at 37°C for 20min, stop the action by adding 500ul 0.4M NaOH, read the OD value at 520nm. Standard curve prepare by double dilute 2uM pyruvate with phosphate bufferr then add 10ul phenylamine citrate and 50ul 2,4-dinitrophenylhydrazine, incubate at 37°C for 20min and record the OD value at 520nm. One unit of AST is the amount of enzyme that will generate 1umole of pyruvate per minute at pH7.4 at 37°C.)
Sequence
Sequence
NCBI and Uniprot Product Information
Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions
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Product Notes
The AST got1 (Catalog #AAA152060) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Aspartate Aminotransferase (AST) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartate Aminotransferase (AST) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the AST got1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartate Aminotransferase (AST), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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