Ebola Virus NP Recombinant Protein | NP recombinant protein

Ebola Virus NP protein

Cat. Num. AAA24009

• Applications: SDS-Page, Western Blot
• Purity: > 90% pure; Purified by chromatograpy from Zaire stain, this nucleoprotein is phosphorylated and O-glycosolated by the host organisms cells.
• Synonyms: Ebola Virus NP; N/A; Ebola Virus NP protein; Ebola Virus Nucleoprotein; Ebola protein; Ebola virus antigen; Zaire Ebola nucleoprotein antigen; Zaire NP protein; Ebola Zaire nucleoprotein antigen; Ebola virus protein; NP recombinant protein

• Purity/Purification: > 90% pure; Purified by chromatograpy from Zaire stain, this nucleoprotein is phosphorylated and O-glycosolated by the host organisms cells.
• Form/Format: Liquid in 50mM Tris,pH 8, 200 mM NaCI, 8M urea, 1 mM EDTA, 2mM DTT.
• Concentration: 1.0 mg/mL (varies by lot)
• Sequence Length: 739

• Applicable Applications for NP recombinant protein: SDS-PAGE, Western Blot (WB)
• Application Notes: 250 ng of Ebola NP protein was loaded onto the gel and transferred to nitrocellulose membrane which was probed with an anti-his antibody at a dilution of 1:1000
• Source: Viral
• Amino Acid Expression Region: 1-739 aa
• Tag/Conjugate: His-Tag
• Protein Type: Recombinant
• Post Translational Modifications: Ebola nucleoprotein is Phosphorylated and O-glycosylated by the host organisms cells.
• Biological Significance: The Ebola Virus (EBOV) causes severe hemorrhagic fever with a mortality rate of up to 90%. EBOV is a member of the order Mononegavirales and, like other viruses in this taxonomic group, contains a negative-sense single-stranded (ss) RNA. The EBOV ssRNA encodes seven distinct proteins. One of them, the nucleoprotein (NP), is the most abundant viral protein in the infected cell and within the viral nucleocapsid. Like other EBOV proteins, NP is multifunctional. It is tightly associated with the viral genome and is essential for viral transcription, RNA replication, genome packaging and nucleocapsid assembly prior to membrane encapsulation.
• Expression System: E. Coli
• Implications in Disease: Typically, Ebola virus infection runs its course within 14 to 21 days. Infection initially presents with nonspecific flu-like symptoms such as fever, myalgia, and malaise. As the infection progresses, patients exhibit severe bleeding and coagulation abnormalities, including gastrointestinal bleeding, rash, and a range of hematological irregularities, such as lymphopenia and neutrophilia. Cytokines are released when reticuloendothelial cells encounter virus, which can contribute to exaggerated inflammatory responses that are not protective. Damage to the liver, combined with massive viremia, leads to disseminated intravascular coagulopathy. The virus eventually infects microvascular endothelial cells and compromises vascular integrity. The terminal stages of Ebola virus infection usually include diffuse bleeding, and hypotensive shock accounts for many Ebola virus fatalities.
• Sequence information: The Ebola nucleoprotein is a homooligomer found on the Ebola virus. It homomultimerizes to form the nucleocapsid. At 739 amino acids, the nucleoprotein (NP) of Ebola virus is the largest nucleoprotein of the nonsegmented negative-stranded RNA viruses, and like the NPs of other viruses, it plays a central role in virus replication. The predicted coding region is 2217 bases in length and encodes a protein that contains 739 amino acids, with a calculated molecular weight of 83.3 kDa. The protein has an approximate net charge of -30 and can be divided into a hydrophobic N-terminal half and a hydrophilic and highly acidic C-terminal half.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Ships on dry ice.; Upon receipt store at -70°C.

WB (Western Blot)

WB (Western Blot)

SDS-PAGE

Product Categories/Family for NP recombinant protein

Infectious Disease; Conjugated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 22789223
• NCBI GeneID: 955194
• NCBI Accession #: NP_690580
• NCBI GenBank Nucleotide #: NC_004161.1
• Molecular Weight: 87 kDa
• NCBI Official Full Name: nucleoprotein
• NCBI Official Symbol: NP
• NCBI Protein Information: nucleoprotein