Ebola Virus VP40 Recombinant Protein

Ebola Virus VP40 protein

Cat. Num. AAA224577

• Applications: SDS-Page, Western Blot, ELISA, Lateral Flow
• Purity: > 90% pure; Purified using proprietary chromatographic techniques
• Synonyms: Ebola Virus VP40; N/A; Ebola Virus VP40 protein; Recombinant Ebola Virus VP40 protein; Ebola Virus VP40 Matrix protein; Ebola VP40 protein; Ebola Zaire protein; Ebola Virus VP40 recombinant protein

• Host: E. coli
• Purity/Purification: > 90% pure; Purified using proprietary chromatographic techniques
• Form/Format: Liquid in 50mM Tris pH 8.5, with 150mM NaCl, 10% glycerol, 1mM EDTA and 2mM DTT.
• Concentration: 1.0 mg/ml (varies by lot)

• Applicable Applications for Ebola Virus VP40 recombinant protein: SDS-PAGE, WB (Western Blot), ELISA, LF (Lateral Flow)
• Grade: Recombinant
• Species: Viral
• Residues: 1-326aa of Ebola VP40 protein with N-terminal Hexahistidine and GST tags. Zaire strain, Accession No. AHX24648.
• Tag/Conjugate: His-GST tag
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Ships on dry ice. Upon receipt, aliquot and store at -70 degree C for long term storage. Avoid repeated freeze/thaw cycles.

WB (Western Blot)

(250 ng (left lane) and 500 ng (right lane) were resolved on an SDS-PAGE and transferred to nitrocellulose membrane. The blot was probed with an anti-his antibody. A strong band of approximately 68 kDa was observed, along with a weaker band of approximately 25 kDa which represents an insignificant amount of degradation product.)

SDS_PAGE

(Left lane: 250 ng VP40 protein; Middle lane: Molecular weight marker; Right lane: 500 ng VP40 protein. Expected molecular weight of 68 kDa.)

WB (Western Blot)

(250 ng (left lane) and 500 ng (right lane) were resolved on an SDS-PAGE and transferred to nitrocellulose membrane. The blot was probed with an anti-his antibody. A strong band of approximately 68 kDa was observed, along with a weaker band of approximately 25 kDa which represents an insignificantamount of degradation product.)

SDS-PAGE

(Left lane: 250 ng VP40 protein; Middle lane: Molecular weight marker; Right lane: 500 ng VP40 protein. Expected molecular weight of 68 kDa.)

WB (Western Blot)

(100 ng of Ebola Virus VP40 protein (AAA224577) was loaded, antibody dilution was 1:3000)

Related Product Information for Ebola Virus VP40 recombinant protein

The Ebola VP40 protein is a viral matrix protein. VP40 coordinates numerous functions in the viral life cycle of the Ebola virus such as,regulation of viral transcription, morphogenesis,andpackaging and budding of mature virions. The VP40 monomer consists of two protein domains, the N-terminal oligomerization domain and the C-terminal membrane-binding domain, connected by a flexible linker. Study of the matrix protein VP40 is important due to the high mortality rate of the Ebola virus, which is listed as a WHO Risk Group 4 Pathogen, an HHS Select Agent, an NIH/NIAID Category A Priority Pathogen, a CDC Category A Bioterrorism Agent, and a Biological Agent for Export Control by the Australia Group.

Product Categories/Family for Ebola Virus VP40 recombinant protein

Infectious Disease