Application Data
(The purity of ERF1 was determined to be >95% by densitometry, approx. MW 74 kDa)
ERF1 recombinant protein
ERF1 Protein
Gene Names
ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
Applications
Western Blot
Purity
>95%
Synonyms
ERF1; N/A; ERF1 Protein; ERF1 recombinant protein
Host
Sf9 insect cells using baculovirus
Purity/Purification
>95%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1ug/ul (varies by lot)
Applicable Applications for ERF1 recombinant protein
Enzyme Assay, Western Blot (WB)
Tag
N-terminal GST
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Dry Ice.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Dry Ice.
Application Data
(The purity of ERF1 was determined to be >95% by densitometry, approx. MW 74 kDa)
Application Data
(The purity of ERF1 was determined to be >95% by densitometry, approx. MW 74 kDa)
Related Product Information for ERF1 recombinant protein
Description: Recombinant human ERF1 (2-end) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: Eukaryotic peptide chain release factor subunit 1 (eRF1) directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Scientific Background: Eukaryotic peptide chain release factor subunit 1 (eRF1) directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Product Categories/Family for ERF1 recombinant protein
References
1. Zilka, N., et al. Truncated tau from sporadic Alzheimer's disease suffices to drive neurofibrillary degeneration in vivo. FEBS Lett. 2006; 508: 3582-3588. 2. Rial, A. et al: Calcium Dyshomeostasis in beta-Amyloid and Tau-bearing Skeletal Myotubes. J. Biol. Chem., 2004; 279: 3524-53532
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens eukaryotic translation termination factor 1 (ETF1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
eukaryotic translation termination factor 1
NCBI Official Symbol
ETF1
NCBI Official Synonym Symbols
ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
NCBI Protein Information
eukaryotic peptide chain release factor subunit 1
UniProt Protein Name
Eukaryotic peptide chain release factor subunit 1
UniProt Gene Name
ETF1
UniProt Synonym Gene Names
ERF1; RF1; SUP45L1; Eukaryotic release factor 1; eRF1
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Product Notes
The ERF1 etf1 (Catalog #AAA14254) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ERF1 can be used in a range of immunoassay formats including, but not limited to, Enzyme Assay, Western Blot (WB). Researchers should empirically determine the suitability of the ERF1 etf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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