Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 ug/mL can bind Anti-GPRC5D recombinant antibody , the EC50 is 8.847-10.38 ng/mL.)
G-protein coupled receptor family C group 5 member D (GPRC5D) Recombinant Protein | GPRC5D recombinant protein
Recombinant Human G-protein coupled receptor family C group 5 member D (GPRC5D)-VLPs (Active)
Purity
The purity information is not available for VLPs proteins.
Synonyms
G-protein coupled receptor family C group 5 member D (GPRC5D); N/A; Recombinant Human G-protein coupled receptor family C group 5 member D (GPRC5D)-VLPs (Active); GPRC5D; G-protein coupled receptor family C group 5 member D; GPRC5D recombinant protein
Host
Mammalian cell
Purity/Purification
The purity information is not available for VLPs proteins.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Sequence Positions
1-345aa; Full Length
Sequence
MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVLPTQLLFLLSVLGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRGCVSFSWTTILCIAIGCSLLQIIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATFCGPCENWKQHGRLIFITVLFSIIIWVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNACPVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV
Species
Homo sapiens (Human)
Tag
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Research Area
Signal TransductionLess than 1.0 EU/ug as determined by LAL method.
Transmembrane Domain
7TM
Biological_Activity
Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 ug/mL can bind Anti-GPRC5D recombinant antibody, the EC50 is 8.847-10.38 ng/mL.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 ug/mL can bind Anti-GPRC5D recombinant antibody , the EC50 is 8.847-10.38 ng/mL.)
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 ug/mL can bind Anti-GPRC5D recombinant antibody , the EC50 is 8.847-10.38 ng/mL.)
Application Data
(is detected by Mouse anti-6*His monoclonal antibody.)
Application Data
(is detected by Mouse anti-6*His monoclonal antibody.)
Related Product Information for GPRC5D recombinant protein
GPRC5D in MM cells makes this gene and its encoded surface protein as promising markers for monitoring the tumor load and hopefully also as targets for antimyeloma antibodies. PMID: 23510526Overexpression of G protein-coupled receptor 5D in the bone marrow is associated with poor prognosis in patients with multiple myeloma. PMID: 22591013
Product Categories/Family for GPRC5D recombinant protein
References
"Cloning and characterization of a human orphan family C G-protein coupled receptor GPRC5D."Braeuner-Osborne H., Jensen A.A., Sheppard P.O., Brodin B., Krogsgaard-Larsen P., O'Hara P.Biochim. Biophys. Acta 1518:237-248(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,791 Da
NCBI Official Full Name
G-protein coupled receptor family C group 5 member D
NCBI Official Synonym Full Names
G protein-coupled receptor, family C, group 5, member D
NCBI Official Symbol
GPRC5D
NCBI Protein Information
G-protein coupled receptor family C group 5 member D; orphan G-protein coupled receptor
UniProt Protein Name
G-protein coupled receptor family C group 5 member D
UniProt Gene Name
GPRC5D
UniProt Entry Name
GPC5D_HUMAN
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Product Notes
The GPRC5D gprc5d (Catalog #AAA279282) is a Recombinant Protein produced from Mammalian cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-345aa; Full Length. The amino acid sequence is listed below: MYKDCIESTG DYFLLCDAEG PWGIILESLA ILGIVVTILL LLAFLFLMRK IQDCSQWNVL PTQLLFLLSV LGLFGLAFAF IIELNQQTAP VRYFLFGVLF ALCFSCLLAH ASNLVKLVRG CVSFSWTTIL CIAIGCSLLQ IIIATEYVTL IMTRGMMFVN MTPCQLNVDF VVLLVYVLFL MALTFFVSKA TFCGPCENWK QHGRLIFITV LFSIIIWVVW ISMLLRGNPQ FQRQPQWDDP VVCIALVTNA WVFLLLYIVP ELCILYRSCR QECPLQGNAC PVTAYQHSFQ VENQELSRAR DSDGAEEDVA LTSYGTPIQP QTVDPTQECF IPQAKLSPQQ DAGGV. It is sometimes possible for the material contained within the vial of "G-protein coupled receptor family C group 5 member D (GPRC5D), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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