Application Data
(The purity of VLPs was greater than 95% as determined by SEC-HPLC.)
Protein HAPLESS 2 (HAP2) Recombinant Protein | HAP2 recombinant protein
Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2)-VLPs
Gene Names
HAP2; GCS1; GENERATIVE CELL-SPECIFIC 1; HAPLESS 2; T5C23.150; T5C23_150
Purity
The purity information is not available for VLPs proteins.
Synonyms
Protein HAPLESS 2 (HAP2); N/A; Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2)-VLPs; GENERATIVE CELL SPECIFIC 1; HAP2 recombinant protein
Host
Mammalian cell
Purity/Purification
The purity information is not available for VLPs proteins.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4.
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4.
Sequence Positions
25-705aa; Full Length of Mature Protein
Sequence
IQILSKSKLEKCEKTSDSGNLNCSTKIVLNLAVPSGSSGGEASIVAEIVEVEDNSSSNMQTVRIPPVITVNKSAAYALYDLTYIRDVPYKPQEYHVTTRKCEHDAGPDIVQICERLRDEKGNVLEQTQPICCPCGPQRRMPSSCGDIFDKMIKGKANTAHCLRFPGDWFHVFGIGQRSLGFSVRVELKTGTRVSEVIIGPENRTATANDNFLKVNLIGDFGGYTSIPSFEDFYLVIPREAAEAGQPGSLGANYSMWMLLERVRFTLDGLECNKIGVGYEAFNTQPNFCSSPYWSCLHNQLWNFRESDINRIDRHQLPLYGLEGRFERINQHPNAGPHSFSIGVTETLNTNLMIELRADDIEYVFQRSPGKIINIAIPTFEALTQFGVAAVIIKNTGEVEASYSLTFDCSKGVAFVEEQFFIIKPKAVTTRSFKLYPTKDQAAKYICTAILKDSQFSEVDRAECQFSTTATVLDNGTQVTNPFQIPETQPKGFFDSIRILWTKIINGLVDFITGDTCRNKCSSFFDFSCHIQYVCLSWMVMFGLLLALFPITCLLLWLLHQKGLFDPCYDWWEDHFDLDHHRRLLPSRADVVNRHHHHHKHRHHHNHHRRTHQRHKHHHGQDDDVLQKMMLERDHSDSHYYHQLHRVHKDSKQKQRRRAKHGIVLPRDVHVERQRKQRLRES
Species
Arabidopsis thaliana (Mouse-ear cress)
Tag
N-terminal Myc-tagged and C-terminal DYKDDDDK-tagged (This tag can be tested only under denaturing conditions)
Research Area
Others
Transmembrane Domain
1TM
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL. Aliquot for long-term storage at -80 degree C.Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
The VLPs are expressed from human 293 cells (HEK293). Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended. Store the protein at -20 degree C/-80 degree C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Application Data
(The purity of VLPs was greater than 95% as determined by SEC-HPLC.)
Application Data
(The purity of VLPs was greater than 95% as determined by SEC-HPLC.)
Application Data
(detected by Mouse anti-6*His monoclonal antibody.)
Application Data
(detected by Mouse anti-6*His monoclonal antibody.)
Related Product Information for HAP2 recombinant protein
Required for male fertility. Plays a role in pollen tube guidance and successful gamete attachment. Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm. Mediates the fusion of cell membranes. Not required for pollen tube outgrowth.
Product Categories/Family for HAP2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,505 Da
NCBI Official Full Name
hapless 2
NCBI Official Symbol
HAP2
NCBI Official Synonym Symbols
GCS1; GENERATIVE CELL-SPECIFIC 1; HAPLESS 2; T5C23.150; T5C23_150
NCBI Protein Information
hapless 2
UniProt Protein Name
Protein HAPLESS 2
UniProt Gene Name
HAP2
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Product Notes
The HAP2 hap2 (Catalog #AAA279278) is a Recombinant Protein produced from Mammalian cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 25-705aa; Full Length of Mature Protein. The amino acid sequence is listed below: IQILSKSKLE KCEKTSDSGN LNCSTKIVLN LAVPSGSSGG EASIVAEIVE VEDNSSSNMQ TVRIPPVITV NKSAAYALYD LTYIRDVPYK PQEYHVTTRK CEHDAGPDIV QICERLRDEK GNVLEQTQPI CCPCGPQRRM PSSCGDIFDK MIKGKANTAH CLRFPGDWFH VFGIGQRSLG FSVRVELKTG TRVSEVIIGP ENRTATANDN FLKVNLIGDF GGYTSIPSFE DFYLVIPREA AEAGQPGSLG ANYSMWMLLE RVRFTLDGLE CNKIGVGYEA FNTQPNFCSS PYWSCLHNQL WNFRESDINR IDRHQLPLYG LEGRFERINQ HPNAGPHSFS IGVTETLNTN LMIELRADDI EYVFQRSPGK IINIAIPTFE ALTQFGVAAV IIKNTGEVEA SYSLTFDCSK GVAFVEEQFF IIKPKAVTTR SFKLYPTKDQ AAKYICTAIL KDSQFSEVDR AECQFSTTAT VLDNGTQVTN PFQIPETQPK GFFDSIRILW TKIINGLVDF ITGDTCRNKC SSFFDFSCHI QYVCLSWMVM FGLLLALFPI TCLLLWLLHQ KGLFDPCYDW WEDHFDLDHH RRLLPSRADV VNRHHHHHKH RHHHNHHRRT HQRHKHHHGQ DDDVLQKMML ERDHSDSHYY HQLHRVHKDS KQKQRRRAKH GIVLPRDVHV ERQRKQRLRE S. It is sometimes possible for the material contained within the vial of "Protein HAPLESS 2 (HAP2), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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