CD226 active protein

CD226 Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA257896

• Gene Names: CD226; PTA1; DNAM1; DNAM-1; TLiSA1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: CD226; N/A; CD226 Protein, Human, Recombinant (ECD, His Tag); Human CD226/DNAM-1 Protein (ECD, His Tag); DNAM-1 Protein, Human; DNAM1 Protein, Human; PTA1 Protein, Human; TLiSA1 Protein, Human; CD226 molecule; CD226 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn247

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2ug/ml (100uL/well) can bind human CD112 with a linear range of 6.4-800ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human DNAM1 (NP_006557.2) extracellular domain (Met1-Asn247) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2 ug/ml (100 ul/well) can bind human CD112 with a linear ranger of 6.4-800 ng/ml.)

SDS-PAGE

Related Product Information for CD226 active protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD226, also known as PTA1 or DNAM-1, is a member of the immunoglobulin superfamily containing 2 Ig-like domains of the V-set. High rate of CD226 (Cluster of Differentiation 226) is found on the surface of natural killer cells, platelets, monocytes and a subset of T cells. CD226 have binding sites with CD112 and CD155 and mediate cellular adhesion to other cells containing its ligands.

Product Categories/Family for CD226 active protein

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.

NCBI and Uniprot Product Information

• NCBI GI #: 171460997
• NCBI GeneID: 10666
• NCBI Accession #: NP_006557.2
• NCBI GenBank Nucleotide #: NM_006566.2
• UniProt Accession #: Q15762; B2R818
• Molecular Weight: 38,614 Da
• NCBI Official Full Name: CD226 antigen
• NCBI Official Synonym Full Names: CD226 molecule
• NCBI Official Symbol: CD226
• NCBI Official Synonym Symbols: PTA1; DNAM1; DNAM-1; TLiSA1
• NCBI Protein Information: CD226 antigen; adhesion glycoprotein; DNAX accessory molecule 1; DNAX accessory molecule-1; platelet and T cell activation antigen 1; T lineage-specific activation antigen 1 antigen
• UniProt Protein Name: CD226 antigen
• UniProt Gene Name: CD226
• UniProt Synonym Gene Names: DNAM1; DNAM-1
• UniProt Entry Name: CD226_HUMAN