LRPAP1 active protein

LRPAP1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA258151

• Gene Names: Lrpap1; RAP; HBP44; C77774; AA617339; AI790446; AU042172
• Purity: >95% as determined by SDS-PAGE
• Synonyms: LRPAP1; N/A; LRPAP1 Protein, Mouse, Recombinant (His Tag); Mouse LRPAP1/RAP Protein (His Tag); AA617339 Protein, Mouse; AI790446 Protein, Mouse; AU042172 Protein, Mouse; C77774 Protein, Mouse; HBP44 Protein, Mouse; RAP Protein, Mouse; low density lipoprotein receptor-related protein associated protein 1; LRPAP1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5% trehalose, 5% mannitol and 0.01% Tween80
• Sequence: Gln29-Leu360

• Species: Mouse
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse LRPAP1 at 0.5ug/ml can bind human VLDLR with a linear range of 1.28-32ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 29
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of mouse LRPAP1 (NP_038615.2) extracellular domain (Gln 29-Leu 360) was fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.
• Reconstitution: It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-PAGE

Related Product Information for LRPAP1 active protein

The recombinant mouse LRPAP1 comprises 343 amino acids with a predicted molecular mass of 40.4 kDa. As a result of glycosylation, it migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.

References

Gonzlez P, et al. (2002) Variation in the lipoprotein receptor-related protein, alpha2-macroglobulin and lipoprotein receptor-associated protein genes in relation to plasma lipid levels and risk of early myocardial infarction. Coron Artery Dis. 13(5): 251-4.Schutte DL, et al. (2003) A LRPAP1 intronic insertion/deletion polymorphism and phenotypic variability in Alzheimer disease. Res Theory Nurs Pract. 17(4): 301-19Pandey SN, et al. (2006) Lipoprotein receptor associated protein (LRPAP1) insertion/deletion polymorphism: association with gallbladder cancer susceptibility. Int J Gastrointest Cancer. 37(4): 124-8.Dixit M, et al. (2006) Association of low density lipoprotein receptor related protein-associated protein (LRPAP1) gene insertion/deletion polymorphism with gallstone disease.J Gastroenterol Hepatol. 21(5): 847-9.

NCBI and Uniprot Product Information

• NCBI GI #: 63999380
• NCBI GeneID: 16976
• NCBI Accession #: NP_038615.2
• NCBI GenBank Nucleotide #: NM_013587.2
• UniProt Accession #: P55302
• NCBI Official Full Name: alpha-2-macroglobulin receptor-associated protein
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein associated protein 1
• NCBI Official Symbol: Lrpap1
• NCBI Official Synonym Symbols: RAP; HBP44; C77774; AA617339; AI790446; AU042172
• NCBI Protein Information: alpha-2-macroglobulin receptor-associated protein
• UniProt Protein Name: Alpha-2-macroglobulin receptor-associated protein
• UniProt Gene Name: Lrpap1
• UniProt Synonym Gene Names: Alpha-2-MRAP; HBP-44; RAP
• UniProt Entry Name: AMRP_MOUSE