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product-image-AAA282498_AD10.jpg Application Data (Recombinant Mouse TNFRSF1B inhibit TNFalpha-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.025-0.1 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNFalpha.)

TNFRSF1B/TNF-R2/CD120b active protein

Active Recombinant Mouse TNFRSF1B/TNF-R2/CD120b Protein

Gene Names
Tnfrsf1b; p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2
Purity
> 95% by SDS-PAGE.
Synonyms
TNFRSF1B/TNF-R2/CD120b; N/A; Active Recombinant Mouse TNFRSF1B/TNF-R2/CD120b Protein; TNFRSF1B; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; TNFRSF1B/TNF-R2/CD120b active protein
Ordering
Host
HEK293 cells
Purity/Purification
> 95% by SDS-PAGE.
Form/Format
Liquid
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Species
Mouse
Tag
C-hFc
Bioactivity
1. Measured by its binding ability in a functional ELISA. Immobilized Mouse TNF-alpha at 2 ug/mL (100 uL/well) can bind Mouse TNFRSF1B with a linear range of 0.02-0.77 ng/mL.
2. Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.025-0.1 ng/mL in the presence of 0.1 ng/mL of Recombinant Mouse TNFalpha.
Preparation and Storage
Store the lyophilized protein at -20 to -80 degree C for 12 months. After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid repeated freeze/thaw cycles.
Ship: Ice Pack

Application Data

(Recombinant Mouse TNFRSF1B inhibit TNFalpha-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.025-0.1 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNFalpha.)

product-image-AAA282498_AD10.jpg Application Data (Recombinant Mouse TNFRSF1B inhibit TNFalpha-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.025-0.1 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNFalpha.)

Application Data

(Immobilized Recombinant Mouse TNF-alpha at 2 ug/mL (100 uL/well) can bind Mouse TNFRSF1B with a linear range of 0.02-0.77 ng/mL)

product-image-AAA282498_AD11.jpg Application Data (Immobilized Recombinant Mouse TNF-alpha at 2 ug/mL (100 uL/well) can bind Mouse TNFRSF1B with a linear range of 0.02-0.77 ng/mL)

Application Data

(The purity of Mouse TNFR2/CD120b/TNFRSF1B Protein (#AAA282498) was greater than 95% as determined by SEC-HPLC.)

product-image-AAA282498_AD13.jpg Application Data (The purity of Mouse TNFR2/CD120b/TNFRSF1B Protein (#AAA282498) was greater than 95% as determined by SEC-HPLC.)

SDS-PAGE

(Active Recombinant Mouse TNFRSF1B/TNFR2/ CD120b Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 65-75kDa.)

product-image-AAA282498_SDS_PAGE15.jpg SDS-PAGE (Active Recombinant Mouse TNFRSF1B/TNFR2/ CD120b Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 65-75kDa.)
Related Product Information for TNFRSF1B/TNF-R2/CD120b active protein
Active Recombinant Mouse TNFRSF1B/TNF-R2/CD120b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val23-Gly258) of mouse TNFR2/CD120b/TNFRSF1B (Accession #NP_035740.2) fused with a hFc tag at the C-terminus.
Product Categories/Family for TNFRSF1B/TNF-R2/CD120b active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,320 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 1B
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 1b
NCBI Official Symbol
Tnfrsf1b
NCBI Official Synonym Symbols
p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2
NCBI Protein Information
tumor necrosis factor receptor superfamily member 1B; TNF receptor beta chain; TNF-RII; TNFR-II; p75 TNFR; p80 TNF-alpha receptor; tumor necrosis factor receptor 2; tumor necrosis factor receptor type II
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 1B
UniProt Gene Name
Tnfrsf1b
UniProt Synonym Gene Names
Tnfr-2; Tnfr2; TNF-R2; TNF-RII; TNFR-II
UniProt Entry Name
TNR1B_MOUSE

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Product Notes

The TNFRSF1B/TNF-R2/CD120b tnfrsf1b (Catalog #AAA282498) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TNFRSF1B/TNF-R2/CD120b, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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