Neuropilin-1 Active Protein | NRP1 active protein

Neuropilin-1 Protein, Mouse, Recombinant (ECD, His Tag)

Cat. Num. AAA258169

• Gene Names: Nrp1; Nrp; NP-1; Npn1; NPN-1; C530029I03
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Neuropilin-1; N/A; Neuropilin-1 Protein, Mouse, Recombinant (ECD, His Tag); Mouse Neuropilin-1/NRP1 Protein (ECD, His Tag); C530029I03 Protein, Mouse; NP-1 Protein, Mouse; NPN-1 Protein, Mouse; Npn1 Protein, Mouse; Nrp Protein, Mouse; neuropilin 1; NRP1 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Phe22-Pro856

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Phe 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse NRP1 (NP_032763.2)(Met1-Pro856) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized VEGF165 at 10ug/mL (100uL/well) can bind mNRP1h. The EC50 of mNRP1h is 30-120ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

SDS-PAGE

(Measured by its binding ability in a functional ELISA. Immobilized VEGF165 can bind mNRP1h, the EC50 of mNRP1h is 30-120 ng/mL.)

Related Product Information for NRP1 active protein

Background: Neuropilin is a type I transmembrane protein and the molecular mass is 120kDa. Two homologs, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins, and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.

References

Nakamura F, et al. (2002) Structural and functional relation of neuropilins. Adv Exp Med Biol. 515: 55-69.Romeo PH, et al. (2002) Neuropilin-1 in the immune system. Adv Exp Med Biol. 515: 49-54.Klagsbrun M, et al. (2002) The role of neuropilin in vascular and tumor biology. Adv Exp Med Biol. 515: 33-48.Staton CA, et al. (2007) Neuropilins in physiological and pathological angiogenesis. J Pathol. 212(3): 237-48.Bagri A, et al. (2009) Neuropilins in tumor biology. Clin Cancer Res. 15(6): 1860-4.

NCBI and Uniprot Product Information

• NCBI GI #: 244792700
• NCBI GeneID: 18186
• NCBI Accession #: NP_032763.2
• NCBI GenBank Nucleotide #: NM_008737.2
• UniProt Accession #: P97333; Q6PAR3
• Molecular Weight: 103,000 Da
• NCBI Official Full Name: neuropilin-1
• NCBI Official Synonym Full Names: neuropilin 1
• NCBI Official Symbol: Nrp1
• NCBI Official Synonym Symbols: Nrp; NP-1; Npn1; NPN-1; C530029I03
• NCBI Protein Information: neuropilin-1
• UniProt Protein Name: Neuropilin-1
• UniProt Gene Name: Nrp1
• UniProt Synonym Gene Names: Nrp