SDS-PAGE
(SDS-PAGE)
TrkB/NTRK2 active protein
Recombinant Human TrkB/NTRK2 Protein (His Tag)(Active)
Gene Names
NTRK2; OBHD; TRKB; trk-B; EIEE58; GP145-TrkB
Applications
SDS-Page
Purity
>95% as determined by reducing SDS-PAGE.
Synonyms
TrkB/NTRK2; N/A; Recombinant Human TrkB/NTRK2 Protein (His Tag)(Active); BDNF/NT-3 Growth Factors Receptor; GP145-TrkB; Trk-B; Neurotrophic Tyrosine Kinase Receptor Type 2; TrkB Tyrosine Kinase; Tropomyosin-Related Kinase B; NTRK2; TRKB ; TrkB/NTRK2 active protein
Host
Human Cells
Purity/Purification
>95% as determined by reducing SDS-PAGE.
Form/Format
Lyophilized from a 0.2 ?m filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Sequence
Cys32-His430
Sequence Length
477
Applicable Applications for TrkB/NTRK2 active protein
SDS-PAGE
Species
Human
Endotoxin
< 1.0 EU per ug as determined by the LAL method.
Tag
C-His
Bio_activity
Immobilized Human NT-3(Cat: MBS2553006) at 2 ug/ml(100 ul/well) can bindHuman TrkB-His. The ED50 of Human TrkB-His is 3.777ug/ml .
Reconstitution
It is recommended that sterile water (200uL) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
Preparation and Storage
Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 degree C to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
SDS-PAGE
(SDS-PAGE)
SDS-PAGE
(SDS-PAGE)
Related Product Information for TrkB/NTRK2 active protein
Protein Construction: Recombinant Human TrkB is produced by our mammalian expression system and the target gene encoding Cys32-His430 is expressed with a 6His tag at the C-terminus.
Background: The TRK Family of Tyrosine Kinase Receptor consists of 3 members: TrkA, TrkB and TrkC. The three TRK family proteins have different ligand specificities. They connect to different neurotrophins, including NGF, BDNF, NT-3NT-4/5. TRKA binds NGF, TRKB binds BDNF and NT-3, TRKC binds NT-4/5. At the protein sequence level, human and rat TRKB have greater than 90% sequence identity and the proteins exbihit cross-species activity. TRKB is primarily expressed in the nervous system and it also expression in a wide variety of tissues with low levels.
Background: The TRK Family of Tyrosine Kinase Receptor consists of 3 members: TrkA, TrkB and TrkC. The three TRK family proteins have different ligand specificities. They connect to different neurotrophins, including NGF, BDNF, NT-3NT-4/5. TRKA binds NGF, TRKB binds BDNF and NT-3, TRKC binds NT-4/5. At the protein sequence level, human and rat TRKB have greater than 90% sequence identity and the proteins exbihit cross-species activity. TRKB is primarily expressed in the nervous system and it also expression in a wide variety of tissues with low levels.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Molecular Mass: 45.25kDa
Actual Protein Molecular Mass: 60-105kDa
Actual Protein Molecular Mass: 60-105kDa
NCBI Official Full Name
BDNF/NT-3 growth factors receptor isoform b
NCBI Official Synonym Full Names
neurotrophic receptor tyrosine kinase 2
NCBI Official Symbol
NTRK2
NCBI Official Synonym Symbols
OBHD; TRKB; trk-B; EIEE58; GP145-TrkB
NCBI Protein Information
BDNF/NT-3 growth factors receptor
UniProt Protein Name
BDNF/NT-3 growth factors receptor
UniProt Gene Name
NTRK2
UniProt Synonym Gene Names
TRKB; Trk-B
UniProt Entry Name
NTRK2_HUMAN
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Product Notes
The TrkB/NTRK2 ntrk2 (Catalog #AAA174698) is an Active Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TrkB/NTRK2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the TrkB/NTRK2 ntrk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Cys32-His4 30. It is sometimes possible for the material contained within the vial of "TrkB/NTRK2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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