Application Data
MLL5 recombinant protein
MLL5 (KMT2E), Active
Gene Names
KMT2E; MLL5; NKp44L; HDCMC04P
Applications
Methyltransferase Assay
Synonyms
MLL5; N/A; MLL5 (KMT2E), Active; MLL5 recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
1-548
Sequence Length
6873
Applicable Applications for MLL5 recombinant protein
Methyltransferase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for MLL5 recombinant protein
Description: Recombinant human MLL5 (1-548) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: Mixed-lineage leukemia protein 5 (MLL5) plays a key regulatory role in hematopoiesis by mediating mono- or demethylation of lysine-4 of histone H3 (1). MLL5 can associate with host cell factor-1 (HCF-1) and be recruited to E2F1-responsive promoters, and thus promotes cell cycle progression (2). Isoform NKp44L acts as cellular ligand for a natural cytotoxicity receptor, which is critical for NK cell-mediated innate immunity (3).
Scientific Background: Mixed-lineage leukemia protein 5 (MLL5) plays a key regulatory role in hematopoiesis by mediating mono- or demethylation of lysine-4 of histone H3 (1). MLL5 can associate with host cell factor-1 (HCF-1) and be recruited to E2F1-responsive promoters, and thus promotes cell cycle progression (2). Isoform NKp44L acts as cellular ligand for a natural cytotoxicity receptor, which is critical for NK cell-mediated innate immunity (3).
Product Categories/Family for MLL5 recombinant protein
References
1. Emerling, B.M., et al. MLL5, a homolog of Drosophila trithorax located within a segment of chromosome band 7q22 implicated in myeloid leukemia. Oncogene. 21 (31):4849-54, 2002. 2. Zhou, P., et al. Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and E2F1-responsive gene expression via association with host cell factor-1 (HCF-1). J Biol Chem. 288 (24):17532-43, 2013. 3. Baychelier, F., et al. Identification of a cellular ligand for the natural cytotoxicity receptor NKp44. Blood. 122 (17):2935-42, 2013.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
100 kDa
NCBI Official Full Name
Homo sapiens lysine methyltransferase 2E (KMT2E), transcript variant 1, mRNA
NCBI Official Synonym Full Names
lysine methyltransferase 2E
NCBI Official Symbol
KMT2E
NCBI Official Synonym Symbols
MLL5; NKp44L; HDCMC04P
NCBI Protein Information
histone-lysine N-methyltransferase 2E
UniProt Protein Name
Histone-lysine N-methyltransferase 2E
UniProt Gene Name
KMT2E
UniProt Synonym Gene Names
MLL5; Lysine N-methyltransferase 2E
Similar Products
Product Notes
The MLL5 kmt2e (Catalog #AAA73024) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-548. AAA Biotech's MLL5 can be used in a range of immunoassay formats including, but not limited to, Methyltransferase Assay. Researchers should empirically determine the suitability of the MLL5 kmt2e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLL5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.