KRAS recombinant protein

Recombinant Human KRAS Protein

Cat. Num. AAA55968

• Gene Names: KRAS; NS; NS3; CFC2; RALD; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
• Applications: SDS-Page, ELISA, Western Blot
• Purity: > 85% as determined by SDS-PAGE.
• Synonyms: KRAS; N/A; Recombinant Human KRAS Protein; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS3; RASK2; KRAS recombinant protein

• Host: E. coli AA 1-188 (P01116).
• Purity/Purification: > 85% as determined by SDS-PAGE.
• Form/Format: Lyophilized ; 58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, pH 7.4 added with 300 mM Imidazole and 15% glycerol.
• Concentration: 0.9 mg/mL (varies by lot)
• Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSED VPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM
• Sequence Length: 188

• Applicable Applications for KRAS recombinant protein: SDS-PAGE, ELISA, WB (Western Blot)
• Source: Human
• Usage: KRAS Protein - Reconstitute at 0.5 mg/mL in 200ul sterile water for short-term storage. After reconstitution with sterile water, if glycerol has no effect on subsequent expiraments, it is recommended to add an equal volume of glycerol for long-term storage. If a different concentration is needed for your purposes please adjust the reconstitution volume as required (Please note: The ion concentration of the final solution will vary according to the volume used.); Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
• Preparation and Storage: Store it under sterile condition s at-20°C upon receiving. Recommend to aliquot theprotein into smaller quantities for optimal storage.**Avoid repeated freeze-thaw cycles.**; ; Stability: The recombinant protein is stable for up to 6 months from date of receipt at -80°C

SDS-PAGE

Related Product Information for KRAS recombinant protein

Background: Like other members of the Ras family, the KRAS protein is a GTPase and is an early player in many signal transduction pathways. KRAS is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. When mutated, KRAS is a oncogene. The protein product of the normal KRAS gene performs an essential function in normal tissue signaling, and the mutation of a KRAS gene is an essential step in the development of many cancers. KRAS acts as a molecular on/off switch, once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors signal, such as c-Raf and PI 3-kinase. KRAS binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP.

NCBI and Uniprot Product Information

• NCBI GI #: 15718761
• NCBI GeneID: 3845
• NCBI Accession #: NP_004976.2
• NCBI GenBank Nucleotide #: NM_004985.4
• UniProt Accession #: P01116; P01118; Q96D10; A8K8Z5; B0LPF9
• Molecular Weight: Predicted/ Observed MW: 48kDa
• NCBI Official Full Name: GTPase KRas isoform b
• NCBI Official Synonym Full Names: KRAS proto-oncogene, GTPase
• NCBI Official Symbol: KRAS
• NCBI Official Synonym Symbols: NS; NS3; CFC2; RALD; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
• NCBI Protein Information: GTPase KRas
• UniProt Protein Name: GTPase KRas
• UniProt Gene Name: KRAS
• UniProt Synonym Gene Names: KRAS2; RASK2
• UniProt Entry Name: RASK_HUMAN