MEK7 recombinant protein

MEK7, Active

Cat. Num. AAA14256

• Gene Names: MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• Applications: Western Blot
• Purity: >70%
• Synonyms: MEK7; N/A; MEK7, Active; MEK7 recombinant protein

• Host: Sf9 insect cells using baculovirus
• Purity/Purification: >70%
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.05ug/ul (varies by lot)
• Sequence Positions: Full length
• Sequence Length: 3446

• Applicable Applications for MEK7 recombinant protein: Kinase Assay, Western Blot (WB)
• Species: Human
• Specific Activity: 1.9nmol/min/mg
• Tag: GST tag
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Application Data

(The purity of MEK7 was determined to be >70% by densitometry, approx. MW~78kDa)

Application Data

Related Product Information for MEK7 recombinant protein

Description: Recombinant full-length human MEK7 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: Mitogen-activated protein kinase 7 (MKK7 or MEK7) is a dual-specificity protein kinase of the STE7 family. Directly phosphorylates the Thr-Pro-Tyr motif located in the activation loop of JNK1/2 and activate the kinases in response to environmental stress, pro-inflammatory cytokines or developmental cues. The activation of MKK7 is mediated by various MAPKKKs, including mixed lineage protein kinases (MLKs) and MAPK/ERK kinase (MEK) kinase (MEKK1).

Product Categories/Family for MEK7 recombinant protein

Enzymes; Active Kinases

References

1. Davis RJ. Signal transduction by the JNK group of MAP kinases. Cell. 103 (2):239-52, 2000. 2. Chang L, et al: Mammalian MAP kinase signalling cascades. Nature. 410 (6824):37-40, 2001. 3. Asaoka Y, et al: Diverse physiological functions of MKK4 and MKK7 during early embryogenesis. J Biochem. 48 (4):393-401, 2010.

NCBI and Uniprot Product Information

• NCBI GI #: 34192881
• NCBI GeneID: 5609
• Molecular Weight: ~78 kDa
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase kinase 7, mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 7
• NCBI Official Symbol: MAP2K7
• NCBI Official Synonym Symbols: MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 7
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• UniProt Gene Name: MAP2K7
• UniProt Synonym Gene Names: JNKK2; MEK7; MKK7; PRKMK7; SKK4; MAP kinase kinase 7; MAPKK 7; MEK 7; SAPK kinase 4; SAPKK-4; SAPKK4; JNK kinase 2; JNKK 2