ERK1 recombinant protein

ERK1, Unactive recombinant protein

Cat. Num. AAA14252

• Gene Names: MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• Applications: Western Blot
• Purity: >85%
• Synonyms: ERK1; N/A; ERK1, Unactive recombinant protein; PRKM3; P44ERK1; P44MAPK; MAPK3; ERK1 recombinant protein

• Purity/Purification: >85%
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.2 ug/ul (varies by lot)
• Sequence Length: 1902

• Applicable Applications for ERK1 recombinant protein: Kinase Assay, Western Blot (WB)
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Shipped on Dry Ice.; Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

(The purity of ERK1 was determined to be >85% by densitometry.Approx. MW 44kDa.)

Related Product Information for ERK1 recombinant protein

Recombinant full-length tag-free human ERK1 was expressed in E. coli cells. (Gene Accesion#: NM_002746)

Scientific Background: ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).

Product Categories/Family for ERK1 recombinant protein

Unactive Kinase; Signaling Proteins - Unactive Kinases

References

1. Boulton, T G. et al: Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase. Biochemistry. 1991 Jan 8;30(1):278-86.

NCBI and Uniprot Product Information

• NCBI GI #: 91718898
• NCBI GeneID: 5595
• NCBI Accession #: NP_002737.2
• NCBI GenBank Nucleotide #: NM_002746
• UniProt Accession #: P27361; Q8NHX1; A8CZ58; B0LPG3
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase 3 (MAPK3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 3
• NCBI Official Symbol: MAPK3
• NCBI Official Synonym Symbols: ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: MAPK3
• UniProt Synonym Gene Names: ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
• UniProt Entry Name: MK03_HUMAN