Mouse anti-Human 8-Oxoguanine DNA Glycosylase Monoclonal Antibody | anti-OGG1 antibody

8-Oxoguanine DNA Glycosylase

Cat. Num. AAA23930

• Gene Names: OGG1; HMMH; MUTM; OGH1; HOGG1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: 8-Oxoguanine DNA Glycosylase, Antibody; 8-Oxoguanine DNA Glycosylase; HMMH; HOGG1; MUTM; OGH1; anti-OGG1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone Number: CPTC-OGG1-1
• Specificity: 8-oxoguanine (8-oxoG), an oxidized form of guanine, is produced by reactive oxygen species in both DNA and nucleotide pools during normal aging. Accumulation of 8-oxoG increases the occurrence of A:T to C:G or G:C to T:A transversionmutations, because 8-oxoG forms a stable basepair with adenine as well as with cytosine. OGG1 (for 8-oxoG DNA glycosylase), also designated MMH, is a DNA repair enzyme that corrects these mutations. Inactivation of the OGG1 gene leads to a mutator phenotype, characterized by the increase in G:C to T:A transversions. The OGG1 gene encodes eight isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria. Guanine is the main target for reactive oxygen species in DNA, and 8-oxoguanine is the most frequent base lesion. Therefore, formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. Furthermore, defects in OGG1 may be a cause of renal cell carcinoma.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 190

• Applicable Applications for anti-OGG1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: Immunohistochemistry (IHC); 1-2ug/ml; 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes; ; Western Blot (WB); 2-4ug/ml; ; Optimal dilution for a specific application should be determined.
• Positive Control: HeLa or Jurkat cells. Kidney, skin or lymph node, Daudi, MDA-MB-231
• Immunogen: Recombinant full-length human OGG1 protein
• Research Areas: Cardiovascular, Infectious Disease, Nuclear Marker
• Cellular Localization: Mitochondrion, Nucleoplasm, Nucleus, Nucleus matrix, Nucleus speckle
• Preparation and Storage: Antibody with azide - store at 2 to 8 degrees C. Antibody without azide - store at -20 to -80 degrees C. Non-hazardous. No MSDS required.

WB (Western Blot)

(Western blot analysis of MDA-MB-231 and Daudi lysates using 8-Oxoguanine DNA Glycosylase Mouse Monoclonal Antibody (CPTC-OGG1-1).)

SDS-PAGE

(SDS-PAGE Analysis Purified 8-oxoguanine Mouse Monoclonal Antibody (CPTC-OGG1-1). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Skin stained with 8-oxoguanine Mouse Monoclonal Antibody (CPTC-OGG1-1).)

References

Cheng, K.C., et al. 1992. J. Biol. Chem. 267: 166-17

NCBI and Uniprot Product Information

• NCBI GI #: 4587158
• NCBI GeneID: 4968
• UniProt Accession #: O15527
• Molecular Weight: 39kDa
• NCBI Official Full Name: OGG1 protein type 1b, partial
• NCBI Official Synonym Full Names: 8-oxoguanine DNA glycosylase
• NCBI Official Symbol: OGG1
• NCBI Official Synonym Symbols: HMMH; MUTM; OGH1; HOGG1
• NCBI Protein Information: N-glycosylase/DNA lyase
• UniProt Protein Name: N-glycosylase/DNA lyase
• UniProt Gene Name: OGG1
• UniProt Synonym Gene Names: MMH; MUTM; OGH1; AP lyase
• UniProt Entry Name: OGG1_HUMAN