Application Data
(Flow Cytometry analysis of intracellular staining in mouse fibroblast cell line L929 using AAA14733 (filled histogram) or with isotype control (open histogram).)
Mouse anti-Human Actin, alpha, Smooth Muscle Monoclonal Antibody | anti-ACTA2 antibody
Actin, alpha, Smooth Muscle (PE)
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Synonyms
Actin, alpha, Smooth Muscle, Antibody; Actin, alpha, Smooth Muscle (PE); Anti -Actin, alpha, Smooth Muscle (PE); anti-ACTA2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9H15 (Formally known as: 1A4)
Specificity
Recognizes human alpha-Smooth Muscle Actin. Species Crossreactivity: Rabbit, Mouse, Rat
Purity/Purification
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Form/Format
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Concentration
0.025mg/mL (varies by lot)
Applicable Applications for anti-ACTA2 antibody
Flow Cytometry (FC)
Application Notes
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry (Intercellular): Neat. 10ul labels up to 1x10e6 cells from human peripheral blood monocytes.
Optimal dilutions to be determined by the researcher.
Flow Cytometry (Intercellular): Neat. 10ul labels up to 1x10e6 cells from human peripheral blood monocytes.
Optimal dilutions to be determined by the researcher.
Immunogen
Synthetic decapeptide corresponding to the N-terminal residues of human alpha-Smooth Muscle Actin.
Hybridoma
Fusion of a mouse myeloma with B cells obtained from a rat immunized with a synthetic decapeptide from the N-terminal of Alpha-Smooth Muscle Actin.
Preparation and Storage
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Application Data
(Flow Cytometry analysis of intracellular staining in mouse fibroblast cell line L929 using AAA14733 (filled histogram) or with isotype control (open histogram).)
Application Data
(Flow Cytometry analysis of intracellular staining in mouse fibroblast cell line L929 using AAA14733 (filled histogram) or with isotype control (open histogram).)
Related Product Information for anti-ACTA2 antibody
Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
Product Categories/Family for anti-ACTA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
actin, alpha 2, smooth muscle, aorta, isoform CRA_a
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
NCBI Protein Information
actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
UniProt Entry Name
ACTA_HUMAN
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Product Notes
The ACTA2 acta2 (Catalog #AAA14733) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Actin, alpha, Smooth Muscle (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Actin, alpha, Smooth Muscle can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). Suitable for use in Flow Cytometry. Other applications not tested. Flow Cytometry (Intercellular): Neat. 10ul labels up to 1x10e6 cells from human peripheral blood monocytes. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the ACTA2 acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, alpha, Smooth Muscle, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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