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WB (Western Blot)
Mouse ALIX/PDCD6IP Monoclonal Antibody | anti-PDCD6IP antibody
Anti-ALIX/PDCD6IP Antibody (Monoclonal, 14D10)
Gene Names
PDCD6IP; AIP1; ALIX; HP95; DRIP4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Immunogen affinity purified.
Synonyms
ALIX/PDCD6IP, Antibody; Anti-ALIX/PDCD6IP Antibody (Monoclonal, 14D10); Programmed cell death 6-interacting protein; PDCD6-interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95; PDCD6IP; AIP1; ALIX; KIAA1375; anti-PDCD6IP antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
14D10
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
751
Applicable Applications for anti-PDCD6IP antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 0.1-0.5ug/ml (Human, Mouse, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat) byHeat
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat) byHeat
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Immunogen
E Coli-derived human PDCD6IP recombinant protein (Position: A2-D330). Human PDCD6IP shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat PDCD6IP, respectively.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
WB (Western Blot)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistchemistry)
IHC (Immunohistchemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IHC (Immunohistochemistry)
IF (Immunofluorescence)
IF (Immunofluorescence)
FCM (Flow Cytometry)
FCM (Flow Cytometry)
Related Product Information for anti-PDCD6IP antibody
Description: Mouse IgG monoclonal antibody for ALIX/PDCD6IP detection. Tested with WB, IHC-P, FCM, ICC/IF in Human; Mouse; Rat.
Background: Programmed cell death 6-interacting protein is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background: Programmed cell death 6-interacting protein is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
References
1. "Entrez Gene: PDCD6IP programmed cell death 6 interacting protein".
2. Vito P, Pellegrini L, Guiet C, D'Adamio L (Feb 1999). "Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction". J Biol Chem 274 (3): 1533-40.
2. Vito P, Pellegrini L, Guiet C, D'Adamio L (Feb 1999). "Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction". J Biol Chem 274 (3): 1533-40.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
programmed cell death 6 interacting protein, isoform CRA_b
NCBI Official Synonym Full Names
programmed cell death 6 interacting protein
NCBI Official Symbol
PDCD6IP
NCBI Official Synonym Symbols
AIP1; ALIX; HP95; DRIP4
NCBI Protein Information
programmed cell death 6-interacting protein
UniProt Protein Name
Programmed cell death 6-interacting protein
UniProt Gene Name
PDCD6IP
UniProt Synonym Gene Names
AIP1; ALIX; KIAA1375; PDCD6-interacting protein
UniProt Entry Name
PDC6I_HUMAN
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Product Notes
The PDCD6IP pdcd6ip (Catalog #AAA19212) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ALIX/PDCD6IP Antibody (Monoclonal, 14D10) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ALIX/PDCD6IP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 0.1-0.5ug/ml (Human, Mouse, Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse, Rat) byHeat ICC: 2ug/ml (Human) IF: 2ug/ml (Human) FC/FACS: 1-3ug/1x106 cells (Human) Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Researchers should empirically determine the suitability of the PDCD6IP pdcd6ip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALIX/PDCD6IP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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