FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with alpha smooth muscle Actin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit alpha Smooth Muscle Actin Monoclonal Antibody | anti-ACTA2 antibody
alpha Smooth Muscle Actin Antibody
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with alpha smooth muscle Actin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with alpha smooth muscle Actin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining alpha smooth muscle Actin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha smooth muscle Actin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha smooth muscle Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha smooth muscle Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha smooth muscle Actin in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha smooth muscle Actin in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha smooth muscle Actin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining alpha smooth muscle Actin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of alpha smooth muscle Actin on different lysates using anti-alpha smooth muscle Actin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: NIH/3T3)
WB (Western Blot)
(Western blot analysis of alpha smooth muscle Actin on different lysates using anti-alpha smooth muscle Actin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: NIH/3T3)
NCBI and Uniprot Product Information
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Product Notes
The ACTA2 acta2 (Catalog #AAA30060) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The alpha Smooth Muscle Actin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's alpha Smooth Muscle Actin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the ACTA2 acta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha Smooth Muscle Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
Is this α-SMA antibody suitable for IHC, IF, or Western blot applications?
Yes, the α-SMA monoclonal antibody is validated for Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot, and Flow Cytometry applications, making it suitable for detecting smooth muscle actin across multiple techniques.
What species does the α-SMA monoclonal antibody detect?
The α-SMA monoclonal antibody recognizes the protein in human, mouse, rat, hamster, bovine, chicken, and equine samples, providing broad species compatibility for diverse research applications.
What is the clone number and isotype of this antibody?
The standard α-SMA monoclonal antibody is clone 1A4 with IgG2a isotype, the most widely used and characterized clone for smooth muscle actin detection.
What dilution is recommended for tissue staining or protein detection?
Recommended dilutions are 1:500–1:1000 for Western Blot and 1:50–1:100 for IHC tissue staining. For immunofluorescence, use 0.034 µg/mL; optimal dilution should be determined for your specific application.
Can this antibody distinguish smooth muscle actin from other actin isoforms?
Yes, the α-SMA antibody clone 1A4 is specifically designed to recognize α-SMA and shows high specificity without significant cross-reaction with β-actin, γ-cytoplasmic actin, or other actin isoforms due to targeted epitope recognition.
What storage conditions are required to maintain stability?
Store the α-SMA antibody at –20°C in glycerol-containing buffer. Avoid freeze-thaw cycles by aliquoting. When stored properly, the antibody remains stable for 12 months.
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