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FCM (Flow Cytometry)
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Rabbit ATP Monoclonal Antibody | anti-ACLY antibody

ATP Citrate Lyase Antibody

Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
ATP, Antibody; ATP Citrate Lyase Antibody; ACL antibody; Acly antibody; ACLY_HUMAN antibody; ATP citrate (pro-S) lyase antibody; ATP citrate lyase antibody; ATP citrate synthase antibody; ATP-citrate (pro-S-)-lyase antibody; ATP-citrate synthase antibody; ATPcitrate synthase antibody; ATPCL antibody; Citrate cleavage enzyme antibody; CLATP antibody; OTTHUMP00000164773 antibody; anti-ACLY antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
ST51-07
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ACLY antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells with ATP citrate lyase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining ATP citrate lyase in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining ATP citrate lyase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ATP citrate lyase citrate lyase antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of ATP citrate lyase on different lysates using anti-ATP citrate lyase antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse kidney Lane 2: Mouse colon Lane 3: MCF-7)

Related Product Information for anti-ACLY antibody
ATP-citrate synthase, also designated ATP-citrate lyase or citrate cleavage enzyme, is a cytoplasmic homotetramer belonging to the succinate/malate CoA ligase family. The gene coding for this protein maps against chromosome 17q12-q21. ATP-citrate synthase catalyses the formation of acetyl-CoA and oxaloacetate from citrate and CoA. This product, Acetyl-CoA, is necessary for both fatty acid and cholesterol biosynthesis. ATP citrate-lyase is important in the biosynthesis of acetylcholine in nervous tissue.
Product Categories/Family for anti-ACLY antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
47
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
120,839 Da
NCBI Official Full Name
ATP-citrate synthase isoform 1
NCBI Official Synonym Full Names
ATP citrate lyase
NCBI Official Symbol
ACLY
NCBI Official Synonym Symbols
ACL; ATPCL; CLATP
NCBI Protein Information
ATP-citrate synthase; ATP citrate synthase; citrate cleavage enzyme; ATP-citrate (pro-S-)-lyase
UniProt Protein Name
ATP-citrate synthase
UniProt Gene Name
ACLY
UniProt Synonym Gene Names
ACL
UniProt Entry Name
ACLY_HUMAN

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Product Notes

The ACLY acly (Catalog #AAA30094) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP Citrate Lyase Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the ACLY acly for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.