Rabbit BCL-XL Monoclonal Antibody | anti-BCL-XL antibody

BCL-XL Antibody

Cat. Num. AAA30008

• Gene Names: BCL2L1; BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: BCL-XL, Antibody; BCL-XL Antibody; Apoptosis regulator Bcl X antibody; Apoptosis regulator Bcl-X antibody; Apoptosis regulator BclX antibody; B cell lymphoma 2 like antibody; B2CL1_HUMAN antibody; Bcl 2 like 1 protein antibody; Bcl X antibody; Bcl xL antibody; BCL XL/S antibody; Bcl xS antibody; Bcl-2-like protein 1 antibody; Bcl2 Like 1 antibody; Bcl2 related gene antibody; Bcl2-L-1 antibody; BCL2L antibody; Bcl2l1 antibody; BCLX antibody; BclXL antibody; BclXs antibody; DKFZp781P2092 antibody; PPP1R52 antibody; Protein phosphatase 1 regulatory subunit 52 antibody; anti-BCL-XL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: SZ3-03
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-BCL-XL antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells with Bcl-XL antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining Bcl-XL in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining Bcl-XL in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining Bcl-XL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.)

Related Product Information for anti-BCL-XL antibody

The Bcl-2 gene was isolated at the chromosomal breakpoint of t (14;18) bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. A second protein, designated Bcl-associated X protein (Bax) p21, has extensive amino acid homology with Bcl-2 and both homodimerizes and heterodimerizes with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Bcl-x, one of several additional proteins with sequence homology to Bcl-2, is expressed as Bcl-xL, a 233 amino acid protein with 43% sequence identity with Bcl-2 that suppresses cell death, and Bcl-xS, a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188) found in Bcl-xL and which functions as a dominant inhibitor of Bcl-2. A further apoptosis-inducing protein, Bad, dimerizes both with Bcl-xL and to a lesser extent with Bcl-2, thus displacing Bax and inducing apoptosis.

Product Categories/Family for anti-BCL-XL antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4502381
• NCBI GeneID: 598
• NCBI Accession #: NP_001182.1
• NCBI GenBank Nucleotide #: NM_001191.2
• UniProt Accession #: Q07817; Q5CZ89; Q5TE65; Q92976; E1P5L6
• Molecular Weight: 26,049 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform 2
• NCBI Official Synonym Full Names: BCL2-like 1
• NCBI Official Symbol: BCL2L1
• NCBI Official Synonym Symbols: BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S
• NCBI Protein Information: bcl-2-like protein 1; apoptosis regulator Bcl-X; protein phosphatase 1, regulatory subunit 52
• UniProt Protein Name: Bcl-2-like protein 1
• UniProt Gene Name: BCL2L1
• UniProt Synonym Gene Names: BCL2L; BCLX; Bcl2-L-1
• UniProt Entry Name: B2CL1_HUMAN