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WB (Western Blot)
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Mouse beta-tubulin Monoclonal Antibody | anti-TUBB antibody

beta-tubulin (HRP Conjugated) Antibody (5G3)

Gene Names
TUBB; M40; TUBB1; TUBB5; OK/SW-cl.56
Reactivity
Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, sheep, Insect, Yeast
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
beta-tubulin, Antibody; beta-tubulin (HRP Conjugated) Antibody (5G3); Tubulin beta chain (Tubulin beta-5 chain); anti-TUBB antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, sheep, Insect, Yeast
Clonality
Monoclonal
Clone Number
5G3
Specificity
Thebeta-tubulin antibody can detects endogenousbeta-tubulin protein.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TUBB antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:3000
IHC: 1:50-1:200
IF: 1:200
Immunogen
Recombinant Protein of beta-tubulin (HRP Conjugated)
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year

WB (Western Blot)

(Western blot analysis of Hela, with ?-tubulin-HRP Conjugated diluted at 1:10,000, 20,000, 40,000, 80,000, 160,000, 320,000.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,?-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,?-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,?-tubulin (HRP Conjugated) Monoclonal Antibody(5G3) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IF (Immunofluorescence)

(Immunofluorescence analysis of Rat-kidney tissue. 1,?-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Mouse-testis tissue. 1,?-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Human-lung-cancer tissue. 1,?-tubulin (HRP Conjugated) Monoclonal Antibody(5G3)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

Product Categories/Family for anti-TUBB antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
444
NCBI Official Full Name
tubulin beta chain
NCBI Official Synonym Full Names
tubulin, beta class I
NCBI Official Symbol
TUBB
NCBI Official Synonym Symbols
M40; TUBB1; TUBB5; OK/SW-cl.56
NCBI Protein Information
tubulin beta chain; beta1-tubulin; beta 5-tubulin; beta-4 tubulin; beta Ib tubulin; class I beta-tubulin; tubulin beta-1 chain; tubulin beta-5 chain; tubulin beta polypeptide; tubulin, beta polypeptide
UniProt Protein Name
Tubulin beta chain
UniProt Gene Name
TUBB
UniProt Synonym Gene Names
TUBB5
UniProt Entry Name
TBB5_HUMAN

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Product Notes

The TUBB tubb (Catalog #AAA30835) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta-tubulin (HRP Conjugated) Antibody (5G3) reacts with Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, sheep, Insect, Yeast and may cross-react with other species as described in the data sheet. AAA Biotech's beta-tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-1:3000 IHC: 1:50-1:200 IF: 1:200. Researchers should empirically determine the suitability of the TUBB tubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.