IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen Lewis B Mouse Monoclonal Antibody (SPM194).)
Mouse Blood Group Antigen Lewis B Monoclonal Antibody | anti-FUT3 antibody
Blood Group Antigen Lewis B Mouse Monoclonal Antibody
Gene Names
FUT3; LE; Les; FT3B; CD174; FucT-III
Reactivity
Human, Guinea Pig
Applications
Immunofluorescence, Immunohistochemistry
Synonyms
Blood Group Antigen Lewis B, Antibody; Blood Group Antigen Lewis B Mouse Monoclonal Antibody; Blood group Lewis alpha-4-fucosyltransferase; Fucosyltransferase 3; Fucosyltransferase III; FucT-III; FUT3; Galactoside 3(4)-L-fucosyltransferase; gastric mucin; leB; lewis antigen system; lewis B blood group antigen; Lewis FT; major airway glycoprotein; anti-FUT3 antibody
Host
Mouse
Reactivity
Human, Guinea Pig
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM194
Specificity
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
361
Applicable Applications for anti-FUT3 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Immunofluorescence (1-3ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Cellular Localization
Goli apparatus, Golgi stock membrane
Immunogen
Mucin isolated from a human ovarian cyst fluid
Positive Control
Colon
Research Area
Cardiovascular
Preparation and Storage
Antibody with azide: store at 2 to 8°C. Non hazardous.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen Lewis B Mouse Monoclonal Antibody (SPM194).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen Lewis B Mouse Monoclonal Antibody (SPM194).)
References
Richman, P.I. et al. 1987. Monoclonal antibodies to human colorectal epithelium: markers for differentiation and tumour characterization. Int. J. Cancer. 39: 317-328. Rouger, P.h., et al, eds. 1987. Proceedings of the first international work- shop on monoclonal antibodies against human red blood cells and related antigens (Paris, 1987). Blood Transfusion Immunohaematology 30: 353- 720. Bara, J. et al. 1988. Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem. J. 254: 185-193. Torrado J, Correa P, Ruiz B, Bernardi P, Zavala D, Bara J. Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102(2):424-30. Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara. Alteration of sialyl Lewis epitope expression in pterygium. J.Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45kDa
NCBI Official Full Name
galactoside 3(4)-L-fucosyltransferase
NCBI Official Synonym Full Names
fucosyltransferase 3 (Lewis blood group)
NCBI Official Symbol
FUT3
NCBI Official Synonym Symbols
LE; Les; FT3B; CD174; FucT-III
NCBI Protein Information
galactoside 3(4)-L-fucosyltransferase
UniProt Protein Name
Galactoside 3(4)-L-fucosyltransferase
UniProt Gene Name
FUT3
UniProt Synonym Gene Names
FT3B; LE; Lewis FT; FucT-III
UniProt Entry Name
FUT3_HUMAN
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Product Notes
The FUT3 fut3 (Catalog #AAA13833) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Blood Group Antigen Lewis B Mouse Monoclonal Antibody reacts with Human, Guinea Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Blood Group Antigen Lewis B can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Immunofluorescence (1-3ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the FUT3 fut3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Blood Group Antigen Lewis B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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