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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using BMI1 Mouse Monoclonal Antibody (BMI1/2823) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human, Mouse BMI1 Monoclonal Antibody | anti-BMI1 antibody
BMI1 (Oncogene and Stem Cell Marker)
Gene Names
BMI1; PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
BMI1, Antibody; BMI1 (Oncogene and Stem Cell Marker); B lymphoma Mo MLV insertion region 1 homolog; BMI1; BMI1 polycomb ring finger oncogene; FLVI2/BMI1; Oncogene BMI1; PCGF4; Polycomb complex protein BMI-1; Polycomb group ring finger 4; RING finger protein 51; RNF51; anti-BMI1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
BMI1/2823
Specificity
The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
326
Applicable Applications for anti-BMI1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: 1-2ug/ml
IF: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
IF: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
HeLa or Jurkat cells. Colon Carcinoma.
Immunogen
Recombinant fragment (around aa 142-326) of human BMI1 protein (exact sequence is proprietary)
Cellular Localization
Nuclear
Hu-chromosome Location
10p12.2.3
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using BMI1 Mouse Monoclonal Antibody (BMI1/2823) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using BMI1 Mouse Monoclonal Antibody (BMI1/2823) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IF (Immunofluorescence)
(Immunofluorescence Analysis of PFA-fixed HeLa cells labeling BMI1 with BMI1 Mouse Monoclonal Antibody (BMI1/2823) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin.)
IF (Immunofluorescence)
(Immunofluorescence Analysis of PFA-fixed HeLa cells labeling BMI1 with BMI1 Mouse Monoclonal Antibody (BMI1/2823) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin.)
SDS-PAGE
(SDS-PAGE Analysis Purified BMI1 Mouse Monoclonal Antibody (BMI1/2823). Confirmation of Integrity and Purity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified BMI1 Mouse Monoclonal Antibody (BMI1/2823). Confirmation of Integrity and Purity of Antibody.)
WB (Western Blot)
(Western Blot Analysis of Human NIH3T3 cell lysate using BMI1 Mouse Monoclonal Antibody (BMI1/2823).)
WB (Western Blot)
(Western Blot Analysis of Human NIH3T3 cell lysate using BMI1 Mouse Monoclonal Antibody (BMI1/2823).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).)
References
Park IK, et al. J Clin Invest2004; 113:175-179.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41kDa
NCBI Official Full Name
polycomb complex protein BMI-1
NCBI Official Synonym Full Names
BMI1 proto-oncogene, polycomb ring finger
NCBI Official Symbol
BMI1
NCBI Official Synonym Symbols
PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1
NCBI Protein Information
polycomb complex protein BMI-1
UniProt Protein Name
Polycomb complex protein BMI-1
UniProt Gene Name
BMI1
UniProt Synonym Gene Names
PCGF4; RNF51
UniProt Entry Name
BMI1_HUMAN
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Product Notes
The BMI1 bmi1 (Catalog #AAA23935) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BMI1 (Oncogene and Stem Cell Marker) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BMI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. WB: 1-2ug/ml IF: 1-2ug/ml Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the BMI1 bmi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMI1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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