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WB (Western Blot)
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BRD9 recombinant antibody

Rabbit anti-BRD9 Recombinant Monoclonal Antibody [BLR153J]

Gene Names
BRD9; PRO9856; LAVS3040
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
BRD9, Recombinant Antibody; Rabbit anti-BRD9 Recombinant Monoclonal Antibody [BLR153J]; bromodomain-containing protein 9; LAVS3040; PRO9856; rhabdomyosarcoma antigen MU-RMS-40.8; sarcoma antigen NY-SAR-29; Bromodomain-containing protein 9; bromodomain containing 9; BRD9 recombinant antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR153J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for BRD9 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Simple Western Size (SW-Size)
Application Notes
WB: 1:1000
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Immunogen
Between 547 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse BRD9 by western blot. Samples: Whole cell lysate (30 ug) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of human BRD9 by western blot. Samples: Whole cell lysate (30 ug) from Jurkat, HEK293T, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of human BRD9 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from Jurkat cells (WT and KO). Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

SWB (Simple Western)

(Detection of human BRD9 by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

Simple Western

(Detection of mouse BRD9 by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848) used at 1:50 and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

IP (Immunoprecipitation)

(Detection of human BRD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2) used for IP at 20 ul/mg lysate. BRD9 was also immunoprecipitated by a previous lot of this antibody (AAA23848 lot 1) and a second antibody against a different epitope of BRD9 . For blotting immunoprecipitated BRD9, AAA23848 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemistry)

(Detection of mouse BRD9 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of human BRD9 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of human BRD9 by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody ((AAA23848 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of human BRD9 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry)

(Detection of mouse BRD9 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for BRD9 recombinant antibody
BRD9 plays a role in chromatin remodeling and regulation of transcription. It acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated. BRD9 is a component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. BRD9 also orchestrates RAD51-RAD54 complex formation and thereby plays a role in homologous recombination [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H8M2].
Product Categories/Family for BRD9 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,763 Da
NCBI Official Full Name
bromodomain-containing protein 9 isoform 1
NCBI Official Synonym Full Names
bromodomain containing 9
NCBI Official Symbol
BRD9
NCBI Official Synonym Symbols
PRO9856; LAVS3040
NCBI Protein Information
bromodomain-containing protein 9; sarcoma antigen NY-SAR-29; rhabdomyosarcoma antigen MU-RMS-40.8
UniProt Protein Name
Bromodomain-containing protein 9
UniProt Gene Name
BRD9
UniProt Entry Name
BRD9_HUMAN

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Product Notes

The BRD9 brd9 (Catalog #AAA23848) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BRD9 Recombinant Monoclonal Antibody [BLR153J] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRD9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Simple Western Size (SW-Size). WB: 1:1000 IP: 6 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the BRD9 brd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRD9, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.