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WB (Western Blot)
(Detection of human BRG1 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, K-562, HEK293T, MCF-7, HeLa, LNCaP, U2OS, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
BRG1 recombinant antibody
Rabbit anti-BRG1 Recombinant Monoclonal Antibody [BLR106H]
Gene Names
SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
BRG1; Monoclonal Recombinant Antibody; Rabbit anti-BRG1 Recombinant Monoclonal Antibody [BLR106H]; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; transcription activator BRG1; protein brahma homolog 1; protein BRG-1; RTPS2; SNF2; SNF2-beta; SNF2L4; SNF2LB; sucrose nonfermenting-like 4; mitotic growth and transcription activator; SWI2; SNF2-like 4; MRD16; nuclear protein GRB1; BAF190; brahma protein-like 1; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BRM/SWI2-related gene 1; CSS4; global transcription activator homologous sequence; homeotic gene regulator; hSNF2b; BAF190A; Transcription activator BRG1; SWI/SNF related; matrix associated; actin dependent regulator of chromatin; subfamily a; member 4; BRG1 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR106H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for BRG1 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500 Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500 Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500 Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500 Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human BRG1 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, K-562, HEK293T, MCF-7, HeLa, LNCaP, U2OS, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human BRG1 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, K-562, HEK293T, MCF-7, HeLa, LNCaP, U2OS, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse BRG1 by western blot. Samples: Whole cell lysate (50 ug) from mIMCD-3 and A20 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse BRG1 by western blot. Samples: Whole cell lysate (50 ug) from mIMCD-3 and A20 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human BRG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1) used for IP at 20 ul/mg lysate. BRG1 was also immunoprecipitated by rabbit anti-BRG1 antibody For blotting immunoprecipitated BRG1, AAA23834 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human BRG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1) used for IP at 20 ul/mg lysate. BRG1 was also immunoprecipitated by rabbit anti-BRG1 antibody For blotting immunoprecipitated BRG1, AAA23834 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human BRG1 in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human BRG1 in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human BRG1 in FFPE HEK293T cells by ICC. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human BRG1 in FFPE HEK293T cells by ICC. Antibody: Rabbit anti-BRG1 recombinant monoclonal antibody (AAA23834 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human BRG1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-BRG1 recombinant monoclonal (AAA23834 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human BRG1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-BRG1 recombinant monoclonal (AAA23834 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for BRG1 recombinant antibody
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. BRG1/SMARCA4 is an ATPase subunit of the SWI/SNF-A chromatin remodeling complex (BAF complex). In BAF complexes, BRG1/SMARCA4 has been shown to interact with BRCA1.
Product Categories/Family for BRG1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
181,261 Da
NCBI Official Full Name
Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 3, mRNA
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
NCBI Official Symbol
SMARCA4
NCBI Official Synonym Symbols
BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
NCBI Protein Information
transcription activator BRG1; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BRM/SWI2-related gene 1; SNF2-beta; SNF2-like 4; brahma protein-like 1; global transcription activator homologous sequence; homeotic gene regulator; mitotic growth
UniProt Protein Name
Transcription activator BRG1
UniProt Gene Name
SMARCA4
UniProt Synonym Gene Names
BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
UniProt Entry Name
SMCA4_HUMAN
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Product Notes
The BRG1 smarca4 (Catalog #AAA23834) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BRG1 Recombinant Monoclonal Antibody [BLR106H] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500 Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500 Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the BRG1 smarca4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRG1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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