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WB (Western Blot)
(Western Blot Analysis of human Small Intestine tissue lysate using CDH17 Mouse Monoclonal Antibody (CDH17/2618).)
Mouse anti-Human Cadherin 17/LI Cadherin Monoclonal Antibody | anti-CDH17 antibody
Cadherin 17/LI Cadherin (Liver-Intestine Marker)
Gene Names
CDH17; HPT1; CDH16; HPT-1
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Synonyms
Cadherin 17/LI Cadherin, Antibody; Cadherin 17/LI Cadherin (Liver-Intestine Marker); BILL-cadherin; Cadherin-17; CDH17; HPT-1 cadherin; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1 (HPT-1); Intestinal peptide-associated transporter HPT-1; LI-cadherin (liver-intestine); Liver Cadherin; Liver-intestine cadherin; anti-CDH17 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
CDH17/2618
Specificity
It recognizes a protein of 120kDa, which is identified as Cadherin 17 (also known as LI Cadherin). The cadherins are a family of Calcium-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. LI-cadherin (for liver-intestine-cadherin) expression is restricted to liver and intestine tissues and is specifically localized to the basolateral domain of hepatocytes and enterocytes.
Form/Format
200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Sequence Length
832
Applicable Applications for anti-CDH17 antibody
Flow Cytometry (FC), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1-2ug/million cells
WB: 2-4 ug/ml
IHC- Formalin-fixed: 1-2ug/ml for 30 mins at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
WB: 2-4 ug/ml
IHC- Formalin-fixed: 1-2ug/ml for 30 mins at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Positive Control
MCF-7, HepG2 or HT29 cells. Human Stomach, Small Intestine, Colon or liver.
Immunogen
Recombinant fragment (around aa 242-418) of human Cadherin 17 protein (CDH17) (exact sequence is proprietary)
Cellular Localization
Cell Membrane
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Non-hazardous. No MSDS required.
Non-hazardous. No MSDS required.
WB (Western Blot)
(Western Blot Analysis of human Small Intestine tissue lysate using CDH17 Mouse Monoclonal Antibody (CDH17/2618).)
WB (Western Blot)
(Western Blot Analysis of human Small Intestine tissue lysate using CDH17 Mouse Monoclonal Antibody (CDH17/2618).)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cadherin 17 (CDH17) Mouse Monoclonal Antibody (CDH17/2618). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cadherin 17 (CDH17) Mouse Monoclonal Antibody (CDH17/2618). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
FCM (Flow Cytometry)
(Flow Cytometric Analysis of MCF-7 cells using Cadherin 17/CDH17 Mouse Monoclonal Antibody (CDH17/2618) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
FCM (Flow Cytometry)
(Flow Cytometric Analysis of MCF-7 cells using Cadherin 17/CDH17 Mouse Monoclonal Antibody (CDH17/2618) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
SDS-PAGE
(SDS-PAGE AnalysisPurified Cadherin 17/CDH17 Mouse Monoclonal Antibody (CDH17/2618). Confirmation of Purity and Integrity of Antibody)
SDS-PAGE
(SDS-PAGE AnalysisPurified Cadherin 17/CDH17 Mouse Monoclonal Antibody (CDH17/2618). Confirmation of Purity and Integrity of Antibody)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17/CDH17 Mouse Monoclonal Antibody (CDH17/2618).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17/CDH17 Mouse Monoclonal Antibody (CDH17/2618).)
References
Berndorff, D., Gessner, R., Kreft, B., Schnoy, N., Lajours-Petter, A.M., Loch, N., Reutter, W., Hortsch, M. and Tauber, R. 1994. Liver-intestine cadherin: molecular cloning and characterization of a novel Ca2+-dependent cell adhesion molecule expressed in liver and intestine. J. Cell Biol. 125: 1353-1369.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
120kDa
NCBI Official Full Name
cadherin-17
NCBI Official Synonym Full Names
cadherin 17
NCBI Official Symbol
CDH17
NCBI Official Synonym Symbols
HPT1; CDH16; HPT-1
NCBI Protein Information
cadherin-17
UniProt Protein Name
Cadherin-17
UniProt Gene Name
CDH17
UniProt Synonym Gene Names
LI-cadherin
UniProt Entry Name
CAD17_HUMAN
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Product Notes
The CDH17 cdh17 (Catalog #AAA23911) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cadherin 17/LI Cadherin (Liver-Intestine Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cadherin 17/LI Cadherin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunohistochemistry (IHC), Western Blot (WB). FC: 1-2ug/million cells WB: 2-4 ug/ml IHC- Formalin-fixed: 1-2ug/ml for 30 mins at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the CDH17 cdh17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cadherin 17/LI Cadherin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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