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ICC (Immunocytochemistry) (ICC staining Calmodulin in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Rabbit Calmodulin Monoclonal Antibody | anti-CALM antibody

Calmodulin Antibody

Gene Names
CALM2; PHKD; CAMII; PHKD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
Calmodulin; Monoclonal Antibody; Calmodulin Antibody; CALM 1 antibody; CALM 2 antibody; CALM 3 antibody; CALM antibody; CALM_HUMAN antibody; CALM1 antibody; CALM2 antibody; Calm3 antibody; CALML2 antibody; calmodulin 1 (phosphorylase kinase; delta) antibody; Calmodulin 2 (phosphorylase kinase; Calmodulin 3 (phosphorylase kinase; Calmodulin antibody; CaM antibody; CAM I antibody; CAM1 antibody; CAM2 antibody; CAM3 antibody; CAMB antibody; CAMC antibody; CAMI antibody; CAMII antibody; CPVT4 antibody; DD132 antibody; FLJ99410 antibody; LP7057 protein antibody; PHKD antibody; PHKD2 antibody; PHKD3 antibody; phosphorylase kinase delta antibody; phosphorylase kinase; delta subunit antibody; anti-CALM antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SJ16-09
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CALM antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

ICC (Immunocytochemistry)

(ICC staining Calmodulin in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Calmodulin in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining Calmodulin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Calmodulin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining Calmodulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Calmodulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calmodulin antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1, 000 dilution.)

WB (Western Blot) (Western blot analysis of Calmodulin on rat brain lysates using anti-Calmodulin antibody at 1/1, 000 dilution.)
Related Product Information for anti-CALM antibody
The level of intracellular calcium is tightly regulated in all eukaryotic cells. A modest increase in this level can result in a myriad of physiological responses, most of which are mediated by calmodulin (CaM), the universal calcium sensor. CaM directly modulates the activity of protein kinases and phosphatases, ion channels and nitric oxide synthetases. It is generally involved in such diverse processes as cell proliferation, endocytosis, cellular adhesion, protein turn over and smooth muscle contraction. CaM (calmodulin) is an acidic protein, 148 amino acids in length, with four helix-loop-helix calcium binding domains. In humans, 3 distinct genes have been identified (CALM1, CALM2 and CALM3); each encoding the identical protein. CALML3 (calmodulin-like 3, or calmodulin-related protein NB-1) shares significant sequence identity with CaM and it is suggested that it may competitively bind CaM substrates. Interestingly, CaM has been shown to associate with the carboxy terminus of the dystrophin gene product, implying that it may regulate its activity.
Product Categories/Family for anti-CALM antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
805
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,838 Da
NCBI Official Full Name
calmodulin
NCBI Official Synonym Full Names
calmodulin 2 (phosphorylase kinase, delta)
NCBI Official Symbol
CALM2
NCBI Official Synonym Symbols
PHKD; CAMII; PHKD2
NCBI Protein Information
calmodulin; caM; LP7057 protein; prepro-calmodulin 2; phosphorylase kinase delta
UniProt Protein Name
Calmodulin
UniProt Gene Name
CALM1
UniProt Synonym Gene Names
CALM; CAM; CAM1; CaM
UniProt Entry Name
CALM_HUMAN

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Product Notes

The CALM calm1 (Catalog #AAA30044) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calmodulin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calmodulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the CALM calm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calmodulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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