Mouse anti-Human Caspase-3 (Pro and Active) Monoclonal Antibody | anti-Casp3 antibody

Caspase-3 (Pro and Active) Monoclonal Antibody

Cat. Num. AAA14867

• Gene Names: Casp3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Apopain; Caspase-3; A830040C14Rik
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Protein G Chromatography
• Synonyms: Caspase-3 (Pro and Active), Antibody; Caspase-3 (Pro and Active) Monoclonal Antibody; Casp3,Cpp32; anti-Casp3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1, Kappa
• Clone Number: ABM1C12
• Purity/Purification: Protein G Chromatography
• Form/Format: Purified; 25 ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
• Sequence Length: 277

• Applicable Applications for anti-Casp3 antibody: Western blot analysis: 2-4 ug/ml; Immunohistochemical analysis: 5-10 Ug/ml; FACS analysis: 0.5-1 ug/10^6 cells
• Antigen: Caspase-3 (Pro and Active)
• Immunogen: Full length recombinant Caspase-3 protein was used as the immunogen for this antibody
• Preparation and Storage: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

FCM (Flow Cytometry)

(Intracellular FLOW cytometric analysis of Caspase 3 (Clone : ABM1C12) inon Jurkat cells using 05 µg/10^6 cells of antibody. Goat anti-mouse PE conjugate was used as secondary antibody. Green represents isotope control, red represents anti-caspase 3 antibody.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of Caspase-3 in human Tonsil tissue using Caspase-3 antibody (Clone: ABM1C12) at 10 µg/ml.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of Caspase-3 in human Small intestine tissue using Caspase-3 antibdoy (Clone: ABM1C12) at 10 ug/mL/)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of Caspase-3 in squamous cell carcinoma of lungs using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of Caspase-3 in normal human spleen tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of Caspase-3 in adenocarcinoma of rectum using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of Caspase-3 in normal human colon tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 ug/ml.)

WB (Western Blot)

(Western blot analysis of Caspase-3. Anti- Caspase-3 (Clone: ABM1C12) was used at 2 ug/ml on Ramos lysates.)

Related Product Information for anti-Casp3 antibody

Caspases are a member of the cysteine-aspartic acid protease family. Caspase-3 (31 kDa) is an executionary caspase which directly cleaves and activates poly(ADP-ribose) polymerase (PARP), sterol regulatory element binding proteins (SREBPs) or it can also interact with other caspases like caspase-6, -7 and -9. Increased levels of caspase-3 are involved in Huntington Disease-associated cell death. Caspase-3 is the principal caspase in mediating the cleavage of amyloid-beta 4A precursor protein (APP), which is related with neuronal death in Alzheimer's disease. Like other caspases, caspase-3 is also synthesized as a zymogen procaspase which is activated by specific proteolytic cleavage. High levels of caspase-3 are observed in lung, spleen, heart, liver and kidney, moderate levels in brain and skeletal muscle, and low in testis.

Product Categories/Family for anti-Casp3 antibody

Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 549806822
• NCBI GeneID: 836
• NCBI Accession #: NP_001271338.1
• NCBI GenBank Nucleotide #: NM_001284409.1
• UniProt Accession #: P42574; O08668; Q8CHV5; Q9QWI4; P70677
• NCBI Official Full Name: caspase-3
• NCBI Official Synonym Full Names: caspase 3
• NCBI Official Symbol: Casp3
• NCBI Official Synonym Symbols: CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Apopain; Caspase-3; A830040C14Rik
• NCBI Protein Information: caspase-3; SREBP cleavage activity 1; caspase 3, apoptosis related cysteine protease; cysteine protease CPP32; protein Yama
• UniProt Protein Name: Caspase-3
• UniProt Gene Name: Casp3
• UniProt Synonym Gene Names: Cpp32; CASP-3; CPP-32; SCA-1
• UniProt Entry Name: CASP3_MOUSE