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ICC (Immunocytochemistry)
(ICC staining Caspase-8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Rabbit anti-Human Caspase-8 Monoclonal Antibody | anti-CASP8 antibody
Caspase-8 Antibody
Gene Names
CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Caspase-8; Monoclonal Antibody; Caspase-8 Antibody; ALPS2B antibody; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody; Apoptotic cysteine protease antibody; Apoptotic protease Mch-5 antibody; Apoptotic protease Mch5 antibody; CAP4 antibody; CASP-8 antibody; CASP8 antibody; CASP8_HUMAN antibody; Caspase 8 antibody; Caspase 8 apoptosis related cysteine peptidase antibody; Caspase-8 subunit p10 antibody; CED 3 antibody; FADD Like ICE antibody; FADD-homologous ICE/CED-3-like protease antibody; FADD-like ICE antibody; FLICE antibody; FLJ17672 antibody; ICE-like apoptotic protease 5 antibody; MACH alpha 1/2/3 protein antibody; MACH antibody; MACH beta 1/2/3/4 protein antibody; MCH5 antibody; MGC78473 antibody; MORT1 associated ced 3 homolog antibody; MORT1-associated CED-3 homolog antibody; OTTHUMP00000163717 antibody; OTTHUMP00000163720 antibody; OTTHUMP00000163724 antibody; OTTHUMP00000163725 antibody; OTTHUMP00000165062 antibody; OTTHUMP00000165063 antibody; OTTHUMP00000165064 antibody; OTTHUMP00000206552 antibody; OTTHUMP00000206582 antibody; anti-CASP8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
SD08-06
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CASP8 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
IHC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining Caspase-8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Caspase-8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Caspase-8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Caspase-8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Caspase-8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Caspase-8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Caspase-8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-8 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Caspase-8 on different lysates using anti-Caspase-8 antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela)
WB (Western Blot)
(Western blot analysis of Caspase-8 on different lysates using anti-Caspase-8 antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela)
Related Product Information for anti-CASP8 antibody
Initiator caspases, which include caspase-8, activate effector caspases by cleaving inactive forms of effector caspases. In the activation cascade responsible for apoptosis induced by TNFRSF1A and mediated by TNFRSF6/FAS, caspase-8 is the most upstream protease. Caspase-8 binds to adaptor molecule FADD, forming an aggregate referred to as death-inducing signaling complex (DISC), which activates caspase-8. The actived protein is released from the complex and further activates downstream apoptotic proteases. Caspase-8, which is a heterodimer consisting of two subunits (p18 and p10), is widely expressed, but is detected at highest levels in peripheral blood leukocytes (PBLs), thymus, liver and spleen. Defects in CASP8, the gene encoding for caspase-8, may cause CASP8D (caspase-8 deficiency disorder), which is characterized by splenomegaly and CD95-induced apoptosis of PBLs, may lead to immunodeficiency due to defects in T lymphocyte, NK cell and B lymphocyte activation.
Product Categories/Family for anti-CASP8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,391 Da
NCBI Official Full Name
caspase-8 isoform C
NCBI Official Synonym Full Names
caspase 8, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP8
NCBI Official Synonym Symbols
CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
NCBI Protein Information
caspase-8; FADD-like ICE; MACH-alpha-1/2/3 protein; apoptotic protease Mch-5; MACH-beta-1/2/3/4 protein; apoptotic cysteine protease; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; FADD-homologous ICE/CED-3-like protease; caspase 8, apopto
UniProt Protein Name
Caspase-8
UniProt Gene Name
CASP8
UniProt Synonym Gene Names
MCH5; CASP-8; FLICE; MACH
UniProt Entry Name
CASP8_HUMAN
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Product Notes
The CASP8 casp8 (Catalog #AAA30210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase-8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the CASP8 casp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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