Rat anti-Mouse CD11b Monoclonal Antibody | anti-CD11b antibody

Rat anti Mouse CD11b:Amethyst Orange

Cat. Num. AAA12285

• Gene Names: Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: CD11b, Antibody; Rat anti Mouse CD11b:Amethyst Orange; CD11b antibody | M1/70.15; anti-CD11b antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: M1/70.15
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Purified IgG conjugated to Amethyst Orange - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD11b antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: Neat to 1/10
• Immunogen: T cell enriched splenocytes from B10 mice.
• Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
• Conjugation: Amethyst Orange
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Application Data

(Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG. Low power)

Application Data

(Published customer image:Rat anti Mouse CD11b antibody, clone M1/70.15 used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.Image caption:ERp57 overexpression reduces axonal)

Application Data

(Published customer image:Rat anti Mouse CD11b antibody, clone M1/70.15 used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.Image caption:Overexpression of ERp57 in transgeni)

Application Data

(Published customer image:AlexaFluor647 conjugated Rat ati mouse CD11b antibody, clone M1/70.15 used for the evaluation of CD11b expression on immortalized murine microglia by flow cytometry.expressionImage caption:IMG cells display)

Application Data

(Published customer image:Rat anti mouse CD11b antibody, clone M1/70.15 used for the assessment of CD11b expression by activated N9 microglial cells using immunofluorescence.Image caption:Localization of CD11b and p-STAT3 immunoreactiv)

Application Data

(Published customer image:Rat anti Mouse CD11b antibody, clone M1/70.15 used for the localization of microglia in murine brain tissue sections by immunofluorescenceImage caption:HCT1026 inhibits MPTP-induced increased Mac-1, PHOX, iNOX)

Application Data

(Published customer image:Rat anti Mouse CD11b antibody, clone M1/70.15 used for the localization of microglia in murine brain tissue sections by immunofluorescenceImage caption:HCT1026 inhibits MPTP-induced increased reactive Mac-1+ m)

Application Data

(Published customer image:Rat anti MouseCD11b antibody, clone M1/70.15 used to identify murine microglia in vitro by immunofluorescence.Image caption:Immunocytochemical characterisation of cultures of mouse microglia. Cultures of micro)

Application Data

(Figure A. FITC conjugated hamster anti mouse CD11c and Alexa647 conjugated rat IgG2b isotype control . Figure B. FITC conjugated hamster anti mouse CD11c and AlexaFluor647 conjugated rat anti mouse CD11b . All)

IF (Immunofluorescence)

(Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody , green in A and Rat anti Mouse CD8 antibody , red in B, Merged image in C)

Related Product Information for anti-CD11b antibody

Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.

The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.

Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982).

Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whiteland et al. 1995).

This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

NCBI and Uniprot Product Information

• NCBI GI #: 124956
• NCBI GeneID: 16409
• UniProt Accession #: P05555; Q8CA73
• Molecular Weight: 115,196 Da
• NCBI Official Full Name: Integrin alpha-M
• NCBI Official Synonym Full Names: integrin alpha M
• NCBI Official Symbol: Itgam
• NCBI Official Synonym Symbols: CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
• NCBI Protein Information: integrin alpha-M; Mac-1 alpha; CD11B (p170); CR-3 alpha chain; macrophage antigen alpha; complement receptor type 3; leukocyte adhesion receptor MO1; CD11 antigen-like family member B; complement component receptor 3 alpha-a; cell surface glycoprotein MAC
• UniProt Protein Name: Integrin alpha-M
• UniProt Gene Name: Itgam
• UniProt Entry Name: ITAM_MOUSE