Mouse anti-Human CD138 Monoclonal Antibody | anti-SDC1 antibody

CD138 Antibody (C-term) (Ascites)

Cat. Num. AAA28633

• Gene Names: SDC1; SDC; CD138; SYND1; syndecan
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Western Blot, Flow Cytometry, Functional Assay
• Synonyms: CD138, Antibody; CD138 Antibody (C-term) (Ascites); Syndecan-1; SYND1; CD138; SDC1; SDC; anti-SDC1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 587CT7.3.6.5
• Specificity: This CD138 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 210-238 amino acids from the C-terminal region of human CD138.
• Form/Format: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Sequence Positions: 210-238
• Sequence Length: 310

• Applicable Applications for anti-SDC1 antibody: Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:500~8000; FC~~1:10~50; IF~~1:10~50
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with U266 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

FCM (Flow Cytometry)

(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)

FCM (Flow Cytometry)

(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)

FCM (Flow Cytometry)

(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of U266 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)

WB (Western Blot)

(CD138 Antibody (C-term)(Ascites) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).)

Related Product Information for anti-SDC1 antibody

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.

Product Categories/Family for anti-SDC1 antibody

Cancer; Metabolism; Neuroscience; Signal transduction

References

Tsai, E.W., et al. Transplantation 90(8):875-881(2010) Hozumi, K., et al. FEBS Lett. 584(15):3381-3385(2010) Zyada, M.M., et al. Ann Diagn Pathol 14(3):153-161(2010) Al-Shibli, K., et al. APMIS 118(5):371-382(2010) Szumilo, J., et al. Folia Histochem. Cytobiol. 47(4):571-578(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 55749480
• NCBI GeneID: 6382
• NCBI Accession #: NP_001006947.1; NP_002988.3
• NCBI GenBank Nucleotide #: NM_001006946.1
• UniProt Accession #: P18827; Q53QV0; Q546D3; Q96HB7; D6W523; AAB21434; AAB23935
• Molecular Weight: 32462
• NCBI Official Full Name: syndecan-1
• NCBI Official Synonym Full Names: syndecan 1
• NCBI Official Symbol: SDC1
• NCBI Official Synonym Symbols: SDC; CD138; SYND1; syndecan
• NCBI Protein Information: syndecan-1
• UniProt Protein Name: Syndecan-1
• UniProt Gene Name: SDC1
• UniProt Synonym Gene Names: SDC; SYND1
• UniProt Entry Name: SDC1_HUMAN