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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Mouse anti-Human CD138 Monoclonal Antibody | anti-SDC1 antibody
CD138 Antibody (C-term) (Ascites)
Gene Names
SDC1; SDC; CD138; SYND1; syndecan
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot, Flow Cytometry, Functional Assay
Synonyms
CD138, Antibody; CD138 Antibody (C-term) (Ascites); Syndecan-1; SYND1; CD138; SDC1; SDC; anti-SDC1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM
Clone Number
587CT7.3.6.5
Specificity
This CD138 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 210-238 amino acids from the C-terminal region of human CD138.
Form/Format
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Sequence Positions
210-238
Sequence Length
310
Applicable Applications for anti-SDC1 antibody
Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:500~8000
FC~~1:10~50
IF~~1:10~50
FC~~1:10~50
IF~~1:10~50
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with U266 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with U266 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
FCM (Flow Cytometry)
(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
FCM (Flow Cytometry)
(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
FCM (Flow Cytometry)
(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
FCM (Flow Cytometry)
(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
FCM (Flow Cytometry)
(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of U266 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
FCM (Flow Cytometry)
(CD138 Antibody (C-term) (Ascites) flow cytometric analysis of U266 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
WB (Western Blot)
(CD138 Antibody (C-term)(Ascites) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).)
WB (Western Blot)
(CD138 Antibody (C-term)(Ascites) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).)
Related Product Information for anti-SDC1 antibody
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
Product Categories/Family for anti-SDC1 antibody
References
Tsai, E.W., et al. Transplantation 90(8):875-881(2010) Hozumi, K., et al. FEBS Lett. 584(15):3381-3385(2010) Zyada, M.M., et al. Ann Diagn Pathol 14(3):153-161(2010) Al-Shibli, K., et al. APMIS 118(5):371-382(2010) Szumilo, J., et al. Folia Histochem. Cytobiol. 47(4):571-578(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32462
NCBI Official Full Name
syndecan-1
NCBI Official Synonym Full Names
syndecan 1
NCBI Official Symbol
SDC1
NCBI Official Synonym Symbols
SDC; CD138; SYND1; syndecan
NCBI Protein Information
syndecan-1
UniProt Protein Name
Syndecan-1
UniProt Gene Name
SDC1
UniProt Synonym Gene Names
SDC; SYND1
UniProt Entry Name
SDC1_HUMAN
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Product Notes
The SDC1 sdc1 (Catalog #AAA28633) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 210-238. The CD138 Antibody (C-term) (Ascites) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD138 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). WB~~1:500~8000 FC~~1:10~50 IF~~1:10~50. Researchers should empirically determine the suitability of the SDC1 sdc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD138, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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