Application Data
(Flow Cytometry analysis of human peripheral blood granulocytes using AAA14803, followed by anti-mouse IgG FITC.)
Mouse anti-Human CD148 Monoclonal Antibody
CD148 (Receptor-type Tyrosine-protein Phosphatase eta, Protein-tyrosine Phosphatase eta, R-PTP-eta, Density-enhanced Phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine Phosphatase Receptor Type J, R-PTP-J, PTPRJ, DEP1)
Reactivity
Human
Applications
Flow Cytometry
Purity
Purified by affinity chromatography.
Synonyms
CD148, Antibody; CD148 (Receptor-type Tyrosine-protein Phosphatase eta, Protein-tyrosine Phosphatase eta, R-PTP-eta, Density-enhanced Phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine Phosphatase Receptor Type J, R-PTP-J, PTPRJ, DEP1); Anti -CD148 (Receptor-type Tyrosine-protein Phosphatase eta, Protein-tyrosine Phosphatase eta, R-PTP-eta, Density-enhanced Phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine Phosphatase Receptor Type J, R-PTP-J, PTPRJ, DEP1); anti-CD148 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
A3
Specificity
Recognizes human CD148.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-CD148 antibody
Flow Cytometry (FC). Other applications have not tested.
Application Notes
Flow Cytometry: <1ug/10e6 cells in 100ul volume or whole blood Optimal dilutions to be determined by the researcher.
Immunogen
PHA-stimulated human PBMC
Grade
Affinity Purified
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Application Data
(Flow Cytometry analysis of human peripheral blood granulocytes using AAA14803, followed by anti-mouse IgG FITC.)
Application Data
(Flow Cytometry analysis of human peripheral blood granulocytes using AAA14803, followed by anti-mouse IgG FITC.)
Related Product Information for anti-CD148 antibody
CD148, a receptor-like protein tyrosine phosphatase also known as human protein tyrosine phosphatase-eta (HPTP-eta) or density-enhanced protein tyrosine phosphatase-1 (DEP-1), is involved in signal transduction in leukocytes and is thought to contribute to mechanisms of cellular differentiation. In lymphoid organs, CD148 was found to be widely expressed on B and T cells, granulocytes, macrophages, certain dendritic cells as well as mature thymocytes. The cellular level of CD148 was increased after in vitro activation of peripheral blood leukocytes. CD148 as a leukocyte activation marker and may be involved in the regulation of T cell activation. Leukocytes expressing CD148 are significantly upregulated in inflamed tissues and that a subset of these cells co-expresses the activation marker CD25. In non-lymphoid tissues, CD148 was found to be present on many epithelial cell types with glandular and/or endocrine differentiation as well as on fibrocytes, melanocytes and Schwann cells. Among non-hematopoietic cells, CD148 is expressed by characteristic types of epithelial and non-epithelial cells. Downregulation of CD148 might promote dedifferentiation and autonomous growth of such cells in malignant tumors.
Product Categories/Family for anti-CD148 antibody
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The CD148 (Catalog #AAA14803) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD148 (Receptor-type Tyrosine-protein Phosphatase eta, Protein-tyrosine Phosphatase eta, R-PTP-eta, Density-enhanced Phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine Phosphatase Receptor Type J, R-PTP-J, PTPRJ, DEP1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD148 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC). Other applications have not tested. Flow Cytometry: <1ug/10e6 cells in 100ul volume or whole blood Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the CD148 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD148, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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