Application Data
(Figure A. FITC conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Rat IgG2b isotype control . Figure B. FITC conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Rat anti Human CD18 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Rat anti-Human CD18 Monoclonal Antibody | anti-CD18 antibody
Rat Anti Human CD18: Amethyst Orange
Use 10ul of the suggested working dilution to label 106 cells in 100ul
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
Application Data
(Figure A. FITC conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Rat IgG2b isotype control . Figure B. FITC conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Rat anti Human CD18 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data
(Figure A. FITC conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Rat IgG2b isotype control . Figure B. FITC conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Rat anti Human CD18 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data
(Phycoerythrin conjugated Rat anti Human CD18 antibody, clone YFC118.3 used to identify canine leukocytes by flow cytometry.Image caption:Gating strategy for leukocyte identification by means of CD18 expression. Gates for granulocytes and monocytes/lymphocytes were identified using lysed canine whole blood (top panel). CD18 expression relative to isotype control (red population), were documented for granulocytes (middle panel, light blue population) and for monocytes/lymphocytes (bottom panel, blue population). The template was subsequently applied in the assessment of leukocyte contamination of the washed platelets samples. FSC: forward scatter, SSC: side scatter.From: Cremer SE, Catalfamo JL, Goggs R, Seemann SE, Kristensen AT, Brooks MB (2019)Proteomic profiling of the thrombin-activated canine platelet secretome (CAPS).PLoS ONE 14(11): e0224891.doi:10.1371/journal.pone.0224891This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Phycoerythrin conjugated Rat anti Human CD18 antibody, clone YFC118.3 used to identify canine leukocytes by flow cytometry.Image caption:Gating strategy for leukocyte identification by means of CD18 expression. Gates for granulocytes and monocytes/lymphocytes were identified using lysed canine whole blood (top panel). CD18 expression relative to isotype control (red population), were documented for granulocytes (middle panel, light blue population) and for monocytes/lymphocytes (bottom panel, blue population). The template was subsequently applied in the assessment of leukocyte contamination of the washed platelets samples. FSC: forward scatter, SSC: side scatter.From: Cremer SE, Catalfamo JL, Goggs R, Seemann SE, Kristensen AT, Brooks MB (2019)Proteomic profiling of the thrombin-activated canine platelet secretome (CAPS).PLoS ONE 14(11): e0224891.doi:10.1371/journal.pone.0224891This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Rat anti Human CD18 antibody,clone YFC118.3 used for flow cytometryImage caption:LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing “high affinity” conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 µg/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.From: Dilek N, Poirier N, Hulin P, Coulon F, Mary C, et al. (2013)Targeting CD28, CTLA-4 and PD-L1 Costimulation Differentially Controls Immune Synapses and Function of Human Regulatory and Conventional T-Cells.PLoS ONE 8(12): e83139.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Rat anti Human CD18 antibody,clone YFC118.3 used for flow cytometryImage caption:LFA-1 activation analyses by Flow Cytometry. (A) Profiles of CD11a, CD18 activation epitope (CD18act, representing “high affinity” conformation) and CD18 (CD18tot) expression by Teff and Treg#1 cells pre-incubated or not with indicated antibodies. Anti-CD28 and anti-CTLA-4 were used at 10 µg/ml. (B) Histograms of Mean Fluorescent Intensity (MFI) of CD11a, CD18act and CD18tot expressed on Teff and Treg#1 cells. Ratio CD18act MFI: CD18tot was established to analyze LFA-1 high affinity conformation in indicated conditions. Data are representative of more than three different experiments. Filled gray, negative control; Red line, Teff and Treg cells alone; Green line, cells with APC; Blue line, cells with APC and anti-CD28; and Black line: cells with APC, anti-CD28 and anti-CTLA-4.From: Dilek N, Poirier N, Hulin P, Coulon F, Mary C, et al. (2013)Targeting CD28, CTLA-4 and PD-L1 Costimulation Differentially Controls Immune Synapses and Function of Human Regulatory and Conventional T-Cells.PLoS ONE 8(12): e83139.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Mouse anti Human CD18 antibody, clone YFC118.3 used for immunofluorescence studies using formalin fixed paraffin embedded material following antigen retrieval with citrate buffer at pH6.0Image caption:Subretinal MPs accumulate on the retinal pigment epithelium in AMDA–D Confocal microscopy of IBA?1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z?stack projection; (C): oblique Z?stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E–G Doubleulabeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA?1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z?stack of a subretinal IBA?1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.Data information: Controls omitting the primary antibody showed no staining apart from the autofluorescence. AZ: atrophic zone; LD: large drusen. Scale bar: 50 ?m (A–D); 10 ?m (E–H).From: Levy O, Calippe B, Lavalette S, Hu SJ, Raoul W, Dominguez E, Housset M, Paques M, Sahel JA, Bemelmans AP, Combadiere C, Guillonneau X, Sennlaub F. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration. EMBO Mol Med. 2015 Jan 20. pii: e201404524.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Mouse anti Human CD18 antibody, clone YFC118.3 used for immunofluorescence studies using formalin fixed paraffin embedded material following antigen retrieval with citrate buffer at pH6.0Image caption:Subretinal MPs accumulate on the retinal pigment epithelium in AMDA–D Confocal microscopy of IBA?1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z?stack projection; (C): oblique Z?stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E–G Doubleulabeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA?1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z?stack of a subretinal IBA?1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.Data information: Controls omitting the primary antibody showed no staining apart from the autofluorescence. AZ: atrophic zone; LD: large drusen. Scale bar: 50 ?m (A–D); 10 ?m (E–H).From: Levy O, Calippe B, Lavalette S, Hu SJ, Raoul W, Dominguez E, Housset M, Paques M, Sahel JA, Bemelmans AP, Combadiere C, Guillonneau X, Sennlaub F. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration. EMBO Mol Med. 2015 Jan 20. pii: e201404524.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(R-phycoerythrin conjugated Rat anti Human CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL.A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages.BMC Infect Dis. 2005 May 24;5:39.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(R-phycoerythrin conjugated Rat anti Human CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL.A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages.BMC Infect Dis. 2005 May 24;5:39.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Application Data
(Figure A. RPE conjugated mouse anti human CD19 and FITC conjugated rat IgG2b isotype control . Figure B. RPE conjugated mouse anti human CD19 and FITC conjugated rat anti human CD18 . All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock .)
Application Data
(Figure A. RPE conjugated mouse anti human CD19 and FITC conjugated rat IgG2b isotype control . Figure B. RPE conjugated mouse anti human CD19 and FITC conjugated rat anti human CD18 . All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock .)
Application Data
(Figure A. RPE conjugated mouse anti human CD19 and Pacific Blue conjugated rat IgG2b isotype control . Figure B. RPE conjugated mouse anti human CD19 and Pacific Blue conjugated rat anti human CD18 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock . Data acquired on the ZE5 cell analyzer.)
Application Data
(Figure A. RPE conjugated mouse anti human CD19 and Pacific Blue conjugated rat IgG2b isotype control . Figure B. RPE conjugated mouse anti human CD19 and Pacific Blue conjugated rat anti human CD18 . All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock . Data acquired on the ZE5 cell analyzer.)
Application Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 and Alexa Fluor 700 conjugated Rat IgG2b isotype control . Figure B . Alexa Fluor 488 conjugated Mouse anti Human CD19 and Alexa Fluor 700 conjugated Rat anti Human CD18 . All experiments performed on Human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Application Data
(Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD19 and Alexa Fluor 700 conjugated Rat IgG2b isotype control . Figure B . Alexa Fluor 488 conjugated Mouse anti Human CD19 and Alexa Fluor 700 conjugated Rat anti Human CD18 . All experiments performed on Human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Application Data
(Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 and Amethyst Orange conjugated Rat IgG2b isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 and Amethyst Orange conjugated Rat anti Human CD18 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
Application Data
(Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 and Amethyst Orange conjugated Rat IgG2b isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 and Amethyst Orange conjugated Rat anti Human CD18 . All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer)
CD18 is an integral membrane glycoprotein of ~95 kDa, also known as the beta 2 chain, of the LFA-1 complex. CD18 links non-covalently to either CD11a, b or c molecules forming the heteromeric LFA-1 complex. CD18 acts as the receptor for ICAM-1 and is important for cell adhesion and cell-cell interactions (Reina &Espel 2017).
Rat anti Human CD18 antibody, clone YFC118.3 demonstrates strong reactivity with leucocytes and is not reactive with platelets.
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Product Notes
The CD18 itgb2 (Catalog #AAA12267) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti Human CD18: Amethyst Orange reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD18 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Max. Dilution: Neat Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD18 itgb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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