Mouse CD3 Monoclonal Antibody | anti-CD3E antibody

CD3 (T-Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA13839

• Gene Names: CD3E; T3E; TCRE; IMD18
• Reactivity: Human, Mouse, Rat
• Applications: Flow Cytometry, Immunofluorescence
• Synonyms: CD3, Antibody; CD3 (T-Cell Marker) Mouse Monoclonal Antibody; CD3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex; anti-CD3E antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG3, kappa
• Clone Number: RIV9
• Specificity: Reacts with five invariable CD3 chains (designated as and) with molecular weight ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.
• Form/Format: 200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
• Sequence Length: 207

• Applicable Applications for anti-CD3E antibody: Functional Studies, Flow Cytometry (FC), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Optimal dilution for a specific application should be determined
• Cellular Localization: Cell Surface and Cytoplasmic
• Immunogen: Human peripheral lymphocytes
• Hu-Chromosome Location: 11q23.3
• Positive Control: Jurkat cells. Tonsil and lymph node
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3e clone RIV9 (green), mounted in EverBrite’ Mounting Medium with DAPI (nuclei, blue).)

IF (Immunofluorescence)

(Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3e Mouse Monoclonal Antibody (RIV9) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red dot (Red).)

FCM (Flow Cytometry)

(Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD3 mouse monoclonal antibody (RIV9) (green))

FCM (Flow Cytometry)

(Flow Cytometric Analysis of Jurkat Cells using CD3e Mouse Monoclonal Antibody (RIV9).Followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red))

SDS-PAGE

(SDS-PAGE AnalysisCD3e Mouse Monoclonal Antibody (RIV9).Confirmation of Integrity and Purity of Antibody)

References

Leerling MF et al. Quality control of anti human CD3 and CD4 monoclonal antibodies. Dev Biol Stand 1990, 71:191-200 Vaessen LM et al. RIV-9: a mouse IgG3 anti-human CD3 monoclonal antibody with strong antigen modulating and T cell eliminating properties. Transplant Proc 1989, 21:1026-7 Jiskoot W et al. Purification and stabilisation of a poorly soluble mouse IgG3 monoclonal antibody. J Immunol Methods 1991, 138(2):181-189 Ceuppens JL and Van Vaeck F. Human T cell activation induced by a monoclonal mouse IgG3 anti-CD3 antibody (RIV9) requires binding of the Fc part of the antibody to the monocytic 72-kDa high-affinity Fc receptor (FcRI). Cell Immunol 1989, 118(1):136-46 Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989 Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995. Alberola-Ila J et al. Stimulation through the TCR/CD3 complex up-regulates the CD2 surface expression on human T lymphocytes. J Immunol 1991, 146(4):108

NCBI and Uniprot Product Information

• NCBI GI #: 4502671
• NCBI GeneID: 916
• NCBI Accession #: NP_000724.1
• NCBI GenBank Nucleotide #: NM_000733.3
• UniProt Accession #: P07766; A8K997
• Molecular Weight: 20kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD3 epsilon chain
• NCBI Official Synonym Full Names: CD3e molecule
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: T3E; TCRE; IMD18
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• UniProt Gene Name: CD3E
• UniProt Synonym Gene Names: T3E
• UniProt Entry Name: CD3E_HUMAN