Application Data
(Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used to identify M2 macrophages in murine adipose tissue by immunofluorescence.Image caption:Expression of M2 markers CD206 and CD301 by ATMs of induced CLS and interstitial macrophages. M2 ATMs outside of CLS are highlighted by arrows. Scale bars?=?50?um.From: Lindhorst A, Raulien N, Wieghofer P, Eilers J, Rossi FMV, Bechmann I, Gericke M.Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages.)
Rat anti-Mouse CD301 Monoclonal Antibody | anti-CD301 antibody
RAT ANTI MOUSE CD301:RPE
ALEXA FLUOR 488, ALEXA FLUOR 647, ALEXA FLUOR 700, Amethyst Blue, Amethyst Orange, Biotin, Low Endotoxin, Purified, RPE
Phosphate buffered saline
0.09% sodium azide (NaN3)
1%
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
Application Data
(Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used to identify M2 macrophages in murine adipose tissue by immunofluorescence.Image caption:Expression of M2 markers CD206 and CD301 by ATMs of induced CLS and interstitial macrophages. M2 ATMs outside of CLS are highlighted by arrows. Scale bars?=?50?um.From: Lindhorst A, Raulien N, Wieghofer P, Eilers J, Rossi FMV, Bechmann I, Gericke M.Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages.)
Application Data
(Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used to identify M2 macrophages in murine adipose tissue by immunofluorescence.Image caption:Expression of M2 markers CD206 and CD301 by ATMs of induced CLS and interstitial macrophages. M2 ATMs outside of CLS are highlighted by arrows. Scale bars?=?50?um.From: Lindhorst A, Raulien N, Wieghofer P, Eilers J, Rossi FMV, Bechmann I, Gericke M.Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages.)
Application Data
(Published customer image:Insert meta-description and applicationsImage caption:Insert image caption)
Application Data
(Published customer image:Insert meta-description and applicationsImage caption:Insert image caption)
Application Data
(Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used for the detection of CD301 positive cells in murine atherosclerotic lesions by immunofluorescence.Image caption:Characterization of the M1 and M2 macrophage subsets in the vulnerable atherosclerotic lesion. (a) Double confocal immunofluorescence of atherosclerotic lesions showing CD68 (macrophage) and CD86 (M1 marker) on top or CD86 and CD301 (M2 marker) in the bottom. Arrows point double positive cells for CD68 and CD86 or CD301. (b) Quantification of lesional CD68-CD86 (top) or CD68-CD301 (bottom) area in the models (n=7-8; with 2-tailed t test). Cast HFD and LCCA LRA CD. LCCA: left common carotid artery; LRA: left renal artery; CD: chow diet; HFD: high fat diet.)
Application Data
(Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used for the detection of CD301 positive cells in murine atherosclerotic lesions by immunofluorescence.Image caption:Characterization of the M1 and M2 macrophage subsets in the vulnerable atherosclerotic lesion. (a) Double confocal immunofluorescence of atherosclerotic lesions showing CD68 (macrophage) and CD86 (M1 marker) on top or CD86 and CD301 (M2 marker) in the bottom. Arrows point double positive cells for CD68 and CD86 or CD301. (b) Quantification of lesional CD68-CD86 (top) or CD68-CD301 (bottom) area in the models (n=7-8; with 2-tailed t test). Cast HFD and LCCA LRA CD. LCCA: left common carotid artery; LRA: left renal artery; CD: chow diet; HFD: high fat diet.)
Application Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power.)
Application Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power.)
Application Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power.)
Application Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power.)
Application Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power.)
Application Data
(Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power.)
Application Data
(Figure A. PE conjugated Rat anti Mouse CD11b and Alexa Fluor 700 conjugated Rat IgG2a isotype control . Figure B. PE conjugated Rat anti Mouse CD11b and Alexa Fluor 700 conjugated Rat anti Mouse CD301 . All experiments performed on mouse peritoneal cells gated on live single monocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data
(Figure A. PE conjugated Rat anti Mouse CD11b and Alexa Fluor 700 conjugated Rat IgG2a isotype control . Figure B. PE conjugated Rat anti Mouse CD11b and Alexa Fluor 700 conjugated Rat anti Mouse CD301 . All experiments performed on mouse peritoneal cells gated on live single monocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data
(Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used for the detection of CD301 positive cells in murine atherosclerotic lesions by immunofluorescence.Image caption:Characterization of the M1 and M2 macrophage subsets in the vulnerable atherosclerotic lesion. (a) Double confocal immunofluorescence of atherosclerotic lesions showing CD68 (macrophage) and CD86 (M1 marker) on top or CD86 and CD301 (M2 marker) in the bottom. Arrows point double positive cells for CD68 and CD86 or CD301. (b) Quantification of lesional CD68-CD86 (top) or CD68-CD301 (bottom) area in the models (n=7-8; with 2-tailed t test). Cast HFD and LCCA LRA CD. LCCA: left common carotid artery; LRA: left renal artery; CD: chow diet; HFD: high fat diet.)
Application Data
(Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used for the detection of CD301 positive cells in murine atherosclerotic lesions by immunofluorescence.Image caption:Characterization of the M1 and M2 macrophage subsets in the vulnerable atherosclerotic lesion. (a) Double confocal immunofluorescence of atherosclerotic lesions showing CD68 (macrophage) and CD86 (M1 marker) on top or CD86 and CD301 (M2 marker) in the bottom. Arrows point double positive cells for CD68 and CD86 or CD301. (b) Quantification of lesional CD68-CD86 (top) or CD68-CD301 (bottom) area in the models (n=7-8; with 2-tailed t test). Cast HFD and LCCA LRA CD. LCCA: left common carotid artery; LRA: left renal artery; CD: chow diet; HFD: high fat diet.)
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Product Notes
The CD301 (Catalog #AAA12294) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RAT ANTI MOUSE CD301:RPE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD301 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min Neat; Max 1/5. Researchers should empirically determine the suitability of the CD301 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD301, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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